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World Journal of Gastroenterology
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1007-9327
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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study
Copyright ©The Author(s) 2025.
World J Gastroenterol. Jan 28, 2025; 31(4): 93179
Published online Jan 28, 2025. doi: 10.3748/wjg.v31.i4.93179
Table 1 The sequences of small hairpin RNAs used in vivo
Parameter
5’ to 3’
RELATarget sequenceGGACCTATGAGACCTTCAAGA
RELA shRNAGGACCTATGAGACCTTCAAGA CGAATCTTGAAGGTCTCATAGGTCC
Control shRNAAAACGTGACACGTTCGGAGAACGAATTCTCCGAACGTGTCACGTTT
shRNA: Small hairpin RNA.
Full Size Table
Table 2 The sequences of single guide RNAs used in vitro
Parameter
5’ to 3’
RELARELA sgRNAGCTTCCGCTACAAGTGCGAGGGG
sgRNA: Single guide ribonucleic acid.
Full Size Table
Table 3 Primary antibody information
Antibody
Source
Lot number
Manufacturer
Reactivity
ATF4Rabbit mAb11815Cell signaling technology, Danvers, MA, United StatesMouse, human
β-actinMouse mAbSc-81178Santa Cruz, Dallas, TX, United StatesMouse, human
CHOPMouse mAbAb11419Abcam, Cambridge, MA, United StatesMouse, human
Cleaved caspase-3Rabbit mAb9664Cell signaling technologyHuman, mouse
GPX4Mouse mAbSc-166437Santa CruzMouse, human
GRP78Rabbit mAbAb108615AbcamHuman, mouse
HNF1αMouse mAbSc-393668Santa CruzMouse, human
HRD1Rabbit pAbPa5-76137Thermo Fisher Scientific, Waltham, MA, United StatesMouse, human
MCADMouse mAbSc-365109Santa CruzMouse, human
MLKLRabbit mAbPa5-34733Thermo Fisher ScientificMouse, human
MTPMouse mAbSc-515742Santa CruzMouse, human
p-MLKLRabbit mAb37333sCell signaling technologyMouse, human
RelAMouse mAbSc-514451Santa CruzMouse, human
SREBP1Mouse mAbMa5-16124Thermo Fisher ScientificMouse, human
CHOP: CCAAT-enhancer-binding protein homologous protein; GPX4: Glutathione peroxidase 4; GRP78: 78-kDa glucose-regulated protein; MTP: Microsomal triglyceride transfer protein; HNF1α: Hepatocyte nuclear factor-1 alpha; HRD1: 3-hydroxy-3-methyl glutaryl coenzyme A reductase degradation 1 homolog; MCAD: Medium chain acyl-CoA dehydrogenase; mAb: Monoclonal antibody; MLKL: Mixed lineage kinase domain-like protein; pAb: Polyclonal antibody; p-MLKL: Phosphorylated mixed lineage kinase domain-like protein; SREBP1: Sterol regulatory element-binding protein 1; ATF4: Activating transcription factor 4; RelA: Nuclear factor kappa B p65.
Full Size Table
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