Melatonin-induced ferroptosis in pancreatic cancer cells by stimulating endoplasmic reticulum stress and inhibiting alanine-serine-cysteine transporter 2-driven glutamine metabolism

doi:10.3748/wjg.v31.i32.108654

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Aug 28, 2025 (publication date) through Aug 27, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 28, 2025; 31(32): 108654
Published online Aug 28, 2025. doi: 10.3748/wjg.v31.i32.108654

Table 1 Protein list

Antibody	Clonality	Species	Dilutions WB/IF	Company
PERK	Polyclonal	Rabbit	1:1000	Affinity, China (AF5304)
P-PERK	Polyclonal	Rabbit	1:1000	Affinity, China (DF7576)
elF2α	Polyclonal	Rabbit	1:1000	Affinity, China (AF6087)
P-elF2α	Polyclonal	Rabbit	1:1000	Affinity, China (AF3087)
ATF4	Polyclonal	Rabbit	1:1000/1:200	Proteintech Group, China (10835-1-AP)
ASCT2	Polyclonal	Rabbit	1:1000	Cell Signaling Technology (CST), United States (D7C12)
GLS1 (KGA/GAC)	Polyclonal	Rabbit	1:25000/1:200	Proteintech Group, China (12855-1-AP)
GCLC	Polyclonal	Rabbit	1:8000	Proteintech Group, China (12601-1-AP)
GSS	Polyclonal	Rabbit	1:4000	Proteintech Group, China (15712-1-AP)
XCT	Monoclonal	Rabbit	1:1000	Abcam, United Kingdom (AB307601)
GPX4	Monoclonal	Rabbit	1:5000/1:400	Abcam, United Kingdom (AB125066)
LC3	Polyclonal	Rabbit	1:5000/1:200	Proteintech Group, China (14600-1-AP)
P62	Monoclonal	Rabbit	1:25000/1:1200	Abcam, United Kingdom (AB109012)
β-actin	Monoclonal	Rabbit	1:25000	Abclonal Technology, China (AC038)

WB: Western blotting; IF: Immunofluorescence; PERK: Protein kinase R-like endoplasmic reticulum kinase; P-PERK: Phosphorylated-protein kinase R-like endoplasmic reticulum kinase; elF2α: Eukaryotic initiation factor 2α; P-elF2α: Phosphorylated-eukaryotic initiation factor 2α; ATF4: Activating transcription factor 4; ASCT2: Alanine-serine-cysteine transporter 2; GLS1: Glutaminase 1; KGA: Kidney-type glutaminase A; GAC: Glutaminase C; GCLC: Glutamate-cysteine ligase catalytic subunit; GSS: Glutathione synthetase; XCT: X-cysteine transporter; GPX4: Glutathione peroxidase 4; LC3: Microtubule-associated protein light chain 3; P62: Sequestosome-1.

Citation: Zhao Q, Zhang H, Wu HM, Yang QY, Zhao H, Kang L, Lv XY. Melatonin-induced ferroptosis in pancreatic cancer cells by stimulating endoplasmic reticulum stress and inhibiting alanine-serine-cysteine transporter 2-driven glutamine metabolism. World J Gastroenterol 2025; 31(32): 108654
URL: https://www.wjgnet.com/1007-9327/full/v31/i32/108654.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i32.108654