Interventional effect of hesperetin on N-methyl-N’-nitro-N-nitrosoguanidine-induced exosomal circ008274 in affecting normal cells to promote gastric carcinogenesis

doi:10.3748/wjg.v31.i16.104920

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Apr 28, 2025 (publication date) through Nov 3, 2025

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World Journal of Gastroenterology

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1007-9327

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Basic Study

World J Gastroenterol. Apr 28, 2025; 31(16): 104920
Published online Apr 28, 2025. doi: 10.3748/wjg.v31.i16.104920

Table 1 Primer sequences

Genes	Primer sequence (5’-3’)
E-cadherin	F: CGAGAGCTACACGTTCACGG
	R: GGGTGTCGAGGGAAAAATAGG
N-cadherin	F: CTCCACTTCCACCTCCACAT
	R: GGACTCGCACCAGGAGTAAT
Vimentin	F: TCAATGTTAAGATGGCCCTTG
	R: TGAGTGGGTATCAACCAGAGG
PCNA	F: AACCTGCAGAGCATGGACTC
	R: TCATTGCCGGCGCATTTTAG
Nanog	F: TTTGTGGGCCTGAAGAAAACT
	R: AGGGCTGTCCTGAATAAGCAG
OCT4	F: GGGAGATTGATAACTGGTGTGTT
	R: GTGTATATCCCAGGGTGATCCTC
GAPDH	F: CTTTGGTATCGTGGAAGGACTC
	R: GTAGAGGCAGGGATGATGTTCT
Si1-circ0008274	F: CAAGAUACAAGAUCUCCCCTT
	R: GGGGAGAUCUUGUAUCUUGTT
Si2-circ0008274	F: GAUACAAGAUCUCCCCAAUTT
	R: AUUGGGGAGAUCUUGUAUCTT
U6	F: CGCTTCGGCAGCACATATAC
	R: TTCACGAATTTGCGTGTCATC
	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTG
	GATACGACAAAATA
MiR-4676-5p	F: AAGCTGAGGAGCCAGTGGTGA
	R: ATCCAGTGCAGGGTCCGAGG
	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTG
	GATACGACTCACTG
MiR-4733-3p	F: AAGCACCCACCAGGTCTAGCA
	R: ATCCAGTGCAGGGTCCGAGG
	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTG
	GATACGACATCCCA
MiR-5089-5p	F: TCACGAGCGTGGGATTTCTGAG
	R: ATCCAGTGCAGGGTCCGAGG
	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTG
	GATACGACGATGCT
MiR-526b-5p	F: ACAGTAGCTCTTGAGGGAAGCAC
	R: ATCCAGTGCAGGGTCCAGGG
	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTG
	GATACGACACAGAA
MiR-578	F: CGCGGCACTTCTTGTGCTCT
	R: ATCCAGTGCAGGGTCCGAGG
	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTG
	GATACGACACAATC
MiR-6077	F: CAGTAGCGGGGAAGAGCTGTAC
	R: ATCCAGTGCAGGGTCCGAGG
	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTG
	GATACGACGAAGGC
MiR-6513-5p	F: ACAGTAGTTTGGGATTGACGCCA
	R: ATCCAGTGCAGGGTCCAGGG
	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTG
	GATACGACAGACAT

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; miR: MicroRNA; OCT4: Octamer-binding transcription factor 4; PCNA: Proliferating cell nuclear antigen.

Full Size Table

Citation: Liang ZF, Xu YM, Song JJ, Gao ZH, Qian H, Xu XZ. Interventional effect of hesperetin on N-methyl-N’-nitro-N-nitrosoguanidine-induced exosomal circ008274 in affecting normal cells to promote gastric carcinogenesis. World J Gastroenterol 2025; 31(16): 104920
URL: https://www.wjgnet.com/1007-9327/full/v31/i16/104920.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i16.104920