ALKBH5 suppresses autophagic flux via N6-methyladenosine demethylation of ZKSCAN3 mRNA in acute pancreatitis

Table 1 Primer sequences for qPCR

Genes	Sequence
ALKBH5	Forward 5’- CTTTGCTTCGGCTGCAAGTT -3’
	Reverse 5’- CCGGCGTTCCTTAATGTCCT -3’
ZKSCAN3	Forward 5’- CAGAGTAGGGTGGAAAGCC -3’
	Reverse 5’- AAGGTATGAAGGTCGGGTG -3’
Primer 1	Forward 5’- CCAGGCGGTTCTATTGC -3’
	Reverse 5’- TGGCTTTCCACCCTACTCT -3’
Primer 2	Forward: 5’- CAGAGTAGGGTGGAAAGCC-3’
	Reverse 5’- AGGTATGAAGGTCGGGTG-3’
Primer 3	Forward 5’- TGGTTCGGGATGGCTAG-3’
	Reverse 5’- AACAGCACTGCCTTGGAG-3’
β-actin	Forward 5’- GTGGCCGAGGACTTTGATTG-3’
	Reverse 5’- CCTGTAACAACGCATCTCATATT-3’

Table 2 m⁶A sites of ZKSCAN3 mRNA

Number	Position	Sequence context	Confidence
1	34	GUGCCCCGCCCCCCGGGGUCGGACUUUCGACACUUUUGUGACUGC	High
2	131	ACAGCUACAGUGAAACGGGAGAACUGCUUGGUUCGGGAUGGCUAG	High
3	180	UCAAGGGAAAGCACAACCUUGGACUCACACUCUGCAGAGGACCAG	Very high
4	198	UUGGACUCACACUCUGCAGAGGACCAGAUGGAGCUACUGGUCAUA	High
5	229	AGCUACUGGUCAUAAAGGUGGAACAAGAAGAGGCCUCCCCCUUGG	Moderate
6	534	GUGGCGCUGCUGGAGUACUUGGACAGGCAGCUGGAUGACACACCU	High
7	589	CAGAUGAUGACGAUGGGCAGGAACUCCUUUGCUCCAAGGCAGUGC	Moderate
8	824	CCCAGUCCUUUCCCCCAGAUGGACAGAGCAGGAUUCAUCUCAGAU	Very high
9	849	GAGCAGGAUUCAUCUCAGAUGAACCUCUACAAAGAUGGAAUGCAG	Moderate
10	906	AGCCUGGUUUCCCUGGAUCAGGACAUGCAGACUAAGGUUAGGGAC	Very high
11	914	UUCCCUGGAUCAGGACAUGCAGACUAAGGUUAGGGACUUGCCUCG	Moderate
12	927	GACAUGCAGACUAAGGUUAGGGACUUGCCUCGAGCUGAAGAAUAC	Very high
13	954	CCUCGAGCUGAAGAAUACAGGGACCAAAAGCCUGAGCAGACAGUG	High
14	971	CAGGGACCAAAAGCCUGAGCAGACAGUGUGCUUCCUGGGUGAAGA	Low
15	993	ACAGUGUGCUUCCUGGGUGAAGACACUGUCCCGAUUCCUACAGGU	Low
16	1347	GAAAAGCCCUACGAGUGUGAUGACUGUGGGAAAACCUUCACUCAG	High
17	1387	CUCAGAGCUGCAGCCUCCUUGAACAUCACAGAAUUCACACUGGGG	Low
18	1471	GGCGUAGCUCACAUCUUCUGAGACAUCAGAGGACCCAUACUGGGG	Moderate
19	1633	GUAGGAUUACAAGCCUUAUUGAACACCAAAAAGUACACACUGGUG	Low
20	1745	GAGAAGACACACGGGGAAGAAAACUUCUGUCACAGUGACCCCUGC	Moderate
21	1803	GUUGGUGUUCAACUGUCAUUGAACUGAAGCCACUCUGUAGUUCUU	High
22	1830	AGCCACUCUGUAGUUCUUAAUGACUGCAGAAGUCAUAGGCUGGGG	Very high
23	1985	ACAAGAGUCCUCACCCAUUGGAACUAAAUGGGCUUCCUGACUGUC	Very high