Mechanistic research: Selenium regulates virulence factors, reducing adhesion ability and inflammatory damage of Helicobacter pylori

Table 1 Primer list

Name	Sequence	Company performing synthesis
CagA	F: ACCCCTAGTCGGTAATG	Shanghai Invitrogen Biotech Co., Ltd.
	R: GCTTTAGCTTCTGATACTGC
VacAs1a	F: GTCAGCATCACACCGCAAC	Shanghai Generay Biotech Co., Ltd.
	R: CTGCTTGAATGCGCCAAAC
16sRNA	F: CTGGAGAGACTAAGCCCTCC	Shanghai Invitrogen Biotech Co., Ltd.
	R: AGGATCAAGGTTTAAGGATT
IL-6	F: GCAGAAAAAGGCAAAGAATC	Wuhan Genecreate Biotech Co., Ltd.
	R: CTACATTTGCCGAAGAGC
IL-8	F: CACCGGAAGGAACCATCTCA	Wuhan Genecreate Biotech Co., Ltd.
	R: TGGCAAAACTGCACCTTCACA
TNF-α	F: TCTTCTCGAACCCCGAGTGA	Wuhan Genecreate Biotech Co., Ltd.
	R: CCTCTGATGGCACCACCAG
GAPDH	F: GGACCTGACCTGCCGTCTAG	Wuhan Genecreate Biotech Co., Ltd.
	R: GTAGCCCAGGATGCCCTTGA
Cag1	F: GCTATGGGGATTGTTGGGATAA	Shanghai Sangon Biotech Co., Ltd.
	R: GCTTCAGTTGGTTCGTTGGTAA
Cag3	F: GACACCTTGAATGTGAATGACAAA	Shanghai Sangon Biotech Co., Ltd.
	R: GTTGTAATACCCATTGACTTGCTCTAA
CagT	F: TCTAAAAAGATTACGCTCATAGGCG	Shanghai Sangon Biotech Co., Ltd.
	R: CTTTGGCTTGCATGTTCAAGTTGCC
CagE	F: GCGATTGTTATTGTGCTTGTAG	Shanghai Sangon Biotech Co., Ltd.
	R: GAAGTGGTTAAAAAATCAATGCCCC
CagM	F: ACAAATACAAAAAAGAAAAAGAGGC	Shanghai Sangon Biotech Co., Ltd.
	R: ATTTTTCAACAAGTTAGAAAAAGCC

16sRNA: 16S ribosomal RNA; CagA: Cytotoxin-associated protein A; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; IL-6: Interleukin 6; TNF-a: Tumor necrosis factor-alpha; VacAs1a: Vacuolating cytotoxin gene As1a.

Table 2 Antibody information

Name	Art. No.	Company
CagA (A-10)	sc-32746	Santa Cruz
VacA	sc-28368	Santa Cruz
m-IgGk BP-HRP	sc-516102	Santa Cruz
GAPDH Ab	AF7021	Affinity Biosciences
Goat anti-rabbit IgG (H + L) HPR	S0001	Affinity Biosciences

CagA: Cytotoxin-associated protein A; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; HPR: Horseradish peroxidase; H: Heavy chain; L: Light chain; m-IgGk BP-HRP: Mouse IgGκ light chain binding protein; VacA Vacuolating cytotoxin gene A.

Table 3 Identified differentially expressed genes (S_2 vs S_0)

Gene_id	Readcount_S_2	Readcount_S_0	Log2 fold change	P value	Padj	Gene name	Description
Gene509	376.5506144	256.0954025	0.55616	0.0066152	0.035684	HPG27_RS02500	Cag pathogenicity island protein Cagl
Gene521	1368.459246	801.0552768	0.77258	5.49E-05	0.00089411	HPG27_RS02560	Type IV secretion system apparatus protein CagT
Gene511	1617.425068	1154.535615	0.48639	0.005033	0.28476	HPG27_RS02510	Type IV secretion system apparatus protein Cag3
Gene540	181710.1299	131892.6179	0.46228	0.0039181	0.023777	HPG27_RS02655	Type IV secretion system oncogenic effector CagA
Gene536	1945.873607	3339.614831	-0.77926	0.00032024	0.0036791	HPG27_RS02635	VirB4 family type IV secretion/conjugal transfer ATPase

Table 4 Identified differentially expressed genes (S_8 vs S_0)

Gene_id	Readcount_S_8	Readcount_S_0	Log2 fold change	P value	Padj	Gene name	Description
Gene509	494.7491661	231.643609	1.0948	1.57E-06	3.04E-05	HPG27_RS02500	Cag pathogenicity island protein Cagl
Gene521	1519.085968	724.4396343	1.0683	2.78E-07	7.47E-06	HPG27_RS02560	Type IV secretion system apparatus protein CagT
Gene511	1562.119955	1044.031209	0.58134	0.0021884	0.010463	HPG27_RS02510	Type IV secretion system apparatus protein Cag3
Gene529	311.1908412	460.4755565	-0.56532	0.0049482	0.020333	HPG27_RS02595	Type IV secretion system apparatus protein CagM