Seropharmalogical effects of Fuzheng Huayu decoction on rat Ito cell morphology and function in culture

Table 1 Effect of drug sera on Ito cell viability (^-x ± s)

Group	n	[3H]Pro (cpm/well)
Controls	4	3610.18 ± 99.47
G	4	6578.13 ± 1690.95a
G	4	9606.33 ± 950.85a
13.8 g	4	4560.26 ± 2026.83

^aP < 0.05 vs controls.

Table 2 Effect of drug sera on Ito cell proliferation (^-x ± s)

Group	n	[3H]TdR (cpm/well)	MTT (OD570)
Controls	4	2864.50 ± 239.15	0.4206 ± 0.016
G	4	1979.65 ± 76.76a	0.4625 ± 0.06
G	4	1882.05 ± 112.08a	0.3475 ± 0.040a
13.8g	4	1781.93 ± 49.69a	0.3268 ± 0.081a

^aP < 0.05, vs controls. MTT: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide.

Table 3 Effect of sera in different proportions to medium on Ito cell proliferation (^-x ± s)

Concentration	n	Controls	Drug sera	Inhibitive rate (%)
5%	4	1481.90 ± 168.03	703.03 ± 138.37b	52.56 ± 9.34
10%	4	2013.88 ± 628.87	637.33 ± 214.53b	68.35 ± 10.68
20%	4	2709.88 ± 788.19	480.20 ± 264.31b	82.27 ± 9.75

^bP < 0.01 vs controls.

Table 4 Influence of drug sera on Ito cell collagen synthesis (%, ^-x ± s)

Concentration	n	Intracellular		Extracellular
		Controls	Drug sera	Controls	Drug sera
5%	6	0.42 ± 0.18	0.26 ± 0.10	4.02 ± 1.63	3.12 ± 30.33
10%	6	0.48 ± 0.16	0.25 ± 0.06a	4.49 ± 1.28	2.71 ± 1.04a
20%	6	0.58 ± 0.26	0.24 ± 0.08a	4.80 ± 1.47	2.59 ± 1.06a

^aP < 0.05 vs controls.