Establishment and application of an experimental model of human fetal hepatocytes for investigation of the protective effects of silybin and polyporus umbellalus polysaccharides

Table 1 Levels of alpha-fetoprotein and transaminase in culture medium at different culture times (x ± s)

	n	Activity (U)
		1 h	16 h	24 h
aFP/ng·L-1	3			18.3 ± 2.0
ALT/IU·L-1	3	8.2 ± 2.3	10.0 ± 1.6	12.0 ± 2.1
AST/IU·L-1	3	10.3 ± 1.6	12.4 ± 1.8	13.5 ± 1.5

Table 2 Alanine aminotransferase and aspartate aminotransferase activity in culture medium of hepatocytes (x ± s)

Group	n	ALT activity (U)			AST activity (U)
		1 h	4 h	16 h	1 h	4 h	16 h
Control	3	40 ± 3.1	63 ± 2.5	71 ± 3.0	108 ± 5.6	165 ± 4.5	178 ± 2.4
PSP (0.5 g·L-1)	3	48 ± 2.2	35 ± 2.5	27 ± 3.4	95 ± 2.8	126 ± 2.3	101 ± 3.1
Silybin (0.5 g·L-1)	3	40 ± 2.5	46 ± 3.0	34 ± 3.3	93 ± 3.2	143 ± 4.0	123 ± 3.8

Table 3 Superoxide dismutase level in the culture medium of hepatocytes (x ± s)

Group	n	SOD (nmol/L)
		1 h	4 h	16 h
Control	3	2.3 ± 0.8	3.0 ± 0.6	3.3 ± 1.2
PSP (0.5 g·L-1)	3	3.1 ± 1.8	4.8 ± 1.6	7.9 ± 2.1
Silybin (0.5 g·L-1)	3	2.4 ± 1.6	3.7 ± 1.1	5.9 ± 0.7