Uptake of bacterial lipopolysaccharide and expression of tumor necrosis factor-&alpha;-mRNA in isolated rat intrahepatic bile duct epithelial cells

doi:10.3748/wjg.v3.i1.3

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Mar 15, 1997 (publication date) through Nov 5, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 15, 1997; 3(1): 3-5
Published online Mar 15, 1997. doi: 10.3748/wjg.v3.i1.3

Table 1 Fluorescein isocyanate fluorescent intensities of TNF-α-mRNA expression in isolated intrahepatic bile duct epithelial cells after incubation with lipopolysaccharides (μFI/μm², ^-x ± s)

	FITC fluorescent intensities
	Cytoplasm	Nuclei
Controls	10.00 ± 8.99	2.20 ± 2.04
LPS incubations
3 h	189.15 ± 21.33^b	64.85 ± 14.99^b
6 h	221.38 ± 22.99^b^d	138.15 ± 36.54^b^d
9 h	170.00 ± 15.25^b	70.42 ± 9.08^b

^bCompared with the controls (P < 0.01);

^dCompared with those of LPS incubations for 3 and 9 h (P < 0.01). FITC: fluorescein isocyanate.

Citation: Chen XM, Han DW, Noguchi K, Tanikawa K. Uptake of bacterial lipopolysaccharide and expression of tumor necrosis factor-α-mRNA in isolated rat intrahepatic bile duct epithelial cells. World J Gastroenterol 1997; 3(1): 3-5
URL: https://www.wjgnet.com/1007-9327/full/v3/i1/3.htm
DOI: https://dx.doi.org/10.3748/wjg.v3.i1.3