First report on establishment and characterization of the extrahepatic cholangiocarcinoma sarcoma cell line CBC2T-2

Table 1 Short tandem repeat profile of tumor tissue and cell line

STR loci	CBC2T-2	Tumor tissue
AMEL	X, X	X, X
D19S433	13	13
D5S818	11, 13	11, 13
D21S11	30, 31.2	30, 31.2
D18S51	13, 13	13, 13
D6S1043	12, 18	12, 18
D3S1358	16, 16	16, 16
D13S317	8, 11	8, 11
D7S820	9, 10	9, 10
D16S539	9	9
CSF1PO	10, 12	10, 12
Penta D	9, 11	9, 11
D2S441	11, 12	11, 12
vWA	17, 18	17, 18
D8S1179	14, 15	14, 15
TPOX	10, 11	10, 11
Penta E	12, 14	12, 14
TH01	9	9
D12S391	19, 20	19, 20
D2S1338	19, 22	19, 22
FGA	23, 23.2	23, 23.2

STR: Short tandem repeat.

Table 2 Comparison of somatic mutant genes detected with the Cancer Gene Census database

Gene name	Chrom	Pos	Ref	Alt	CGC-cancers
ALK	Chr2	29221210	G	T	Neuroblastoma
BARD1	Chr2	214809500	G	A	Ovariancancer; breastcancer; endometrioid cancer
KDR	Chr4	55106807	T	A	Melanoma
FAT1	Chr4	186621601	T	C	Pancreatic
WRN	Chr8	31167138	T	C	Osteosarcoma; meningioma; other tumour types
HNF1A	Chr12	120999418	T	C	Hepatic adenoma; hepatocellular carcinoma
BRCA1	Chr17	43071077	T	C	Breast; ovarian
BRCA1	Chr17	43092418	T	C	Breast; ovarian
AXIN2	Chr17	65535650	G	A	Colorectal carcinoma
SETBP1	Chr18	44876688	G	A	Neuroepithelial tumours

Chrom: Chromosome; Pos: Position on chromosome; Ref: Reference base on the genome; Alt: Mutated base; CGC-Cancers: Tumour types with driver genes included in the Cancer Gene Census database.

Table 3 Results of screening for known driver genes

Gene name	Chrom	Pos	Ref	Alt
ARID1A	Chr1	26780184	-	A
SFPQ	Chr1	35192164	G	C
DNMT3A	Chr2	25244384	G	A
CAD	Chr2	27237546	G	A
FBXO11	Chr2	47839654	C	T
LRP1B	Chr2	140683563	C	T
NEB	Chr2	151529162	G	A
NEB	Chr2	151540532	G	A
HOXD13	Chr2	176093701	C	T
MECOM	Chr3	169112718	C	A
MECOM	Chr3	169112734	C	G
ETV5	Chr3	186081304	G	C
NSD2	Chr4	1955726	C	T
NSD1	Chr5	177135451	G	C
AKAP9	Chr7	92097135	G	A
KMT2C	Chr7	152182489	G	A
BRD3	Chr9	134040128	C	T
FAS	Chr10	89014231	G	C
KIF20B	Chr10	89738070	C	G
NUP98	Chr11	3683422	G	T
NUP98	Chr11	3683472	C	T
TNKS1BP1	Chr11	57317951	T	C
KMT2D	Chr12	49040044	G	A
NAV3	Chr12	78159123	C	A
BTG1	Chr12	92145413	G	C
SRCAP	Chr16	30698165	G	A
CNOT1	Chr16	58538927	G	T
CNOT1	Chr16	58576526	C	T
TP53	Chr17	7661876	C	T
TP53	Chr17	7675217	T	C
PRKACA	Chr19	14092743	G	C
ASXL1	Chr20	32359040	C	G
CLTCL1	Chr22	19210202	G	A
MYH9	Chr22	36321935	G	C
DMD	ChrX	33077488	C	T
KDM5C	ChrX	53211367	C	T

Chrom: Chromosome; Pos: Position on chromosome; Ref: Reference base on the genome; Alt: Mutated base.