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©The Author(s) 2022.
World J Gastroenterol. Oct 28, 2022; 28(40): 5845-5864
Published online Oct 28, 2022. doi: 10.3748/wjg.v28.i40.5845
Published online Oct 28, 2022. doi: 10.3748/wjg.v28.i40.5845
Table 1 Quantitative polymerase chain reaction human and mouse primer sequences
| Gene | Primer sequences, 5’ to 3’ | Annealing temperature, °C |
| hCx43 | F: CTTCACTACTTTTAAGCAAAAGAG | 52 |
| R: TCCCTCCAGCAGTTGAG | ||
| hTET-1 | F: TTCGTCACTGCCAACCTTAG | 60 |
| R: ATGCCTCTTTCACTGGGTG | ||
| hTET-2 | F: CACTGCATGTTTGGACTTCTG | 60 |
| R: TGCTCATCCTCAGGTTTTCC | ||
| hTET-3 | F: GCCCACAAGGACCAGCATAA | 60 |
| R: CGCAGCGATTGTCTTCCTTG | ||
| hGAPDH | F: TGGTGCTCAGTGTAGCCCAG | 52-62 |
| R: GGACCTGACCTGCCGTCTAG | ||
| mCx43 | F: ACAGCGGTTGAGTCAGCTTG | 60 |
| R: GAGAGATGGGGAAGGACTTGT | ||
| mTET-2 | F: CAAGGTGTGCTTGGCAATTTT | 60 |
| R: CATCACTGTGCGTCAATCAAGA | ||
| mIl-1β | F: CTCCACCTCAATGGACAGAA | 57 |
| R: GCCGTCTTTCATTACACAGG | ||
| mTNF-α | F: AATGGCCTCCCTCTCATCAGT | 57 |
| R: CCACTTGGTGGTTTGCTACGA | ||
| mGAPDH | F: CATGGCCTTCCGTGTTCCTA | 58 |
| R: CCTGCTTCACCACCTTCTTGAT |
- Citation: El-Harakeh M, Saliba J, Sharaf Aldeen K, Haidar M, El Hajjar L, Awad MK, Hashash JG, Shirinian M, El-Sabban M. Expression of the methylcytosine dioxygenase ten-eleven translocation-2 and connexin 43 in inflammatory bowel disease and colorectal cancer. World J Gastroenterol 2022; 28(40): 5845-5864
- URL: https://www.wjgnet.com/1007-9327/full/v28/i40/5845.htm
- DOI: https://dx.doi.org/10.3748/wjg.v28.i40.5845