Novel therapeutic diiminoquinone exhibits anticancer effects on human colorectal cancer cells in two-dimensional and three-dimensional in vitro models

doi:10.3748/wjg.v28.i33.4787

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Sep 7, 2022 (publication date) through Nov 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 7, 2022; 28(33): 4787-4811
Published online Sep 7, 2022. doi: 10.3748/wjg.v28.i33.4787

Table 1 List of primary and secondary antibodies used in immunofluorescent staining

Antibody name	Species	Dilution	Catalog number	Company
Primary antibodies
CK8	Mouse	1:200	904801	Biolegend
CK19	Rabbit	1:200	ab15463	Abcam
CD44	Mouse	1:100	sc-7297	Santa Cruz Biotechnology
Ki67	Mouse	1:50	sc-23900	Santa Cruz Biotechnology
p- HistoneH2AX	Rabbit	1:200	#9718S	Cell Signaling
Secondary antibodies
Alexa fluoro 488	Goat anti-mouse	1:200	A-28175	Invitrogen
Alexa fluoro 568	Goat anti-rabbit	1:200	A-11011	Invitrogen
Phalloidin		1:200	R415	Invitrogen

Table 2 List of primary and secondary antibodies used in western blot experiments

Antibody name	Species	Dilution	Catalog number	Company
Primary antibodies
p53	Rabbit	1:50	sc-6243	Santa Cruz Biotechnology
p21	Rabbit	1:1000	sc-2947	Cell Signaling
CD133	Rabbit	1:500	#64326S	Cell Signaling
β-catenin	Mouse	1:200	sc-7963	Santa Cruz Biotechnology
PCNA	Mouse	1:50	sc-25280	Santa Cruz Biotechnology
p-ERK	Mouse	1:300	sc-7383	Santa Cruz Biotechnology
ERK	Rabbit	1:300	sc-93	Santa Cruz Biotechnology
p-AKT	Rabbit	1:1000	#4060	Cell Signaling
AKT	Rabbit	1:1000	#4691	Cell Signaling
GAPDH-HRP (6C5)	Mouse	1:20,000	#MAB5476	Abnova
Secondary antibodies
Goat anti-mouse	Goat	1:1000	sc-516102	Santa Cruz Biotechnology
Mouse anti-rabbit	Mouse	1:1000	sc-2357	Santa Cruz Biotechnology

Citation: Monzer A, Wakimian K, Ballout F, Al Bitar S, Yehya A, Kanso M, Saheb N, Tawil A, Doughan S, Hussein M, Mukherji D, Faraj W, Gali-Muhtasib H, Abou-Kheir W. Novel therapeutic diiminoquinone exhibits anticancer effects on human colorectal cancer cells in two-dimensional and three-dimensional in vitro models. World J Gastroenterol 2022; 28(33): 4787-4811
URL: https://www.wjgnet.com/1007-9327/full/v28/i33/4787.htm
DOI: https://dx.doi.org/10.3748/wjg.v28.i33.4787