Copyright
©The Author(s) 2022.
World J Gastroenterol. Sep 7, 2022; 28(33): 4787-4811
Published online Sep 7, 2022. doi: 10.3748/wjg.v28.i33.4787
Published online Sep 7, 2022. doi: 10.3748/wjg.v28.i33.4787
Table 1 List of primary and secondary antibodies used in immunofluorescent staining
| Antibody name | Species | Dilution | Catalog number | Company |
| Primary antibodies | ||||
| CK8 | Mouse | 1:200 | 904801 | Biolegend |
| CK19 | Rabbit | 1:200 | ab15463 | Abcam |
| CD44 | Mouse | 1:100 | sc-7297 | Santa Cruz Biotechnology |
| Ki67 | Mouse | 1:50 | sc-23900 | Santa Cruz Biotechnology |
| p- HistoneH2AX | Rabbit | 1:200 | #9718S | Cell Signaling |
| Secondary antibodies | ||||
| Alexa fluoro 488 | Goat anti-mouse | 1:200 | A-28175 | Invitrogen |
| Alexa fluoro 568 | Goat anti-rabbit | 1:200 | A-11011 | Invitrogen |
| Phalloidin | 1:200 | R415 | Invitrogen | |
Table 2 List of primary and secondary antibodies used in western blot experiments
| Antibody name | Species | Dilution | Catalog number | Company |
| Primary antibodies | ||||
| p53 | Rabbit | 1:50 | sc-6243 | Santa Cruz Biotechnology |
| p21 | Rabbit | 1:1000 | sc-2947 | Cell Signaling |
| CD133 | Rabbit | 1:500 | #64326S | Cell Signaling |
| β-catenin | Mouse | 1:200 | sc-7963 | Santa Cruz Biotechnology |
| PCNA | Mouse | 1:50 | sc-25280 | Santa Cruz Biotechnology |
| p-ERK | Mouse | 1:300 | sc-7383 | Santa Cruz Biotechnology |
| ERK | Rabbit | 1:300 | sc-93 | Santa Cruz Biotechnology |
| p-AKT | Rabbit | 1:1000 | #4060 | Cell Signaling |
| AKT | Rabbit | 1:1000 | #4691 | Cell Signaling |
| GAPDH-HRP (6C5) | Mouse | 1:20,000 | #MAB5476 | Abnova |
| Secondary antibodies | ||||
| Goat anti-mouse | Goat | 1:1000 | sc-516102 | Santa Cruz Biotechnology |
| Mouse anti-rabbit | Mouse | 1:1000 | sc-2357 | Santa Cruz Biotechnology |
- Citation: Monzer A, Wakimian K, Ballout F, Al Bitar S, Yehya A, Kanso M, Saheb N, Tawil A, Doughan S, Hussein M, Mukherji D, Faraj W, Gali-Muhtasib H, Abou-Kheir W. Novel therapeutic diiminoquinone exhibits anticancer effects on human colorectal cancer cells in two-dimensional and three-dimensional in vitro models. World J Gastroenterol 2022; 28(33): 4787-4811
- URL: https://www.wjgnet.com/1007-9327/full/v28/i33/4787.htm
- DOI: https://dx.doi.org/10.3748/wjg.v28.i33.4787
