HP0953 - hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium

Table 1 Prediction of glycosylation sites

Position		Residue score	Prediction
Probable N-linked glycosylation sites
68	NNK	-0.20374993	Non-glycosylated
69	NKY	-0.11718906	Non-glycosylated
80	NAK	-0.46398761	Non-glycosylated
113	NIS	0.031759513	Potentially glycosylated
120	NYL	-0.12578944	Non-glycosylated
130	NTY	-0.42088241	Non-glycosylated
134	NLK	-0.028092183	Non-glycosylated
164	NLN	0.45527702	Potentially glycosylated
166	NDN	-0.14904342	Non-glycosylated
168	NEI	0.2906731	Potentially glycosylated
Probable O-linked glycosylation sites
15	S	0.3493683	Potentially glycosylated
16	S	-0.20711247	Non-glycosylated
18	S	-0.033164353	Non-glycosylated
20	S	-0.066886402	Non-glycosylated
23	S	0.33910492	Potentially glycosylated
33	S	-0.58418843	Non-glycosylated
40	S	-0.76207501	Non-glycosylated
43	S	0.16899016	Potentially glycosylated
78	T	-0.75721234	Non-glycosylated
84	S	-0.20144186	Non-glycosylated
107	S	-0.2527357	Non-glycosylated

There are three probable N-linked and six probable O-linked glycosylation sites.

Table 2 Prediction of posttranslational modification sites

Position	Residue	Prediction
PS00008 MYRISTYL N-myristoylation site
12-17	GVfsSV	N-myristoylation site
29-34	GilvSD	N-myristoylation site
73-78	GIeqAT	N-myristoylation site
182-187	GSvcAL	N-myristoylation site
PS00005 PKC_PHOSPHO_SITE Protein Kinase C phosphorylation site
20-22	SaK	MOD_RES 20 phosphoserine [condition: S]
PS00001 ASN_GLYCOSYLATION N-glycosylation site
113-116	NISY	CARBOHYD 113 N-linked (GlcNAc) asparagine [condition: N]
PS00294 PRENYLATION Prenyl group binding site (CAAX box)
185-188	CALr	Prenyl group binding site (CAAX box)

There are four predicted myristoylation sites, one predicted protein kinase C phosphorylation site, one predicted glycosylation site, and one predicted prenylation site.