Long noncoding RNA TNFRSF10A-AS1 promotes colorectal cancer through upregulation of HuR

Table 1 Primers for real-time polymerase chain reaction

Primer name	Forward (5’-3’)	Reverse (5’-3’)
TNFRSF10A-AS1	TCTCAGATCACGTGACCTTGA	GTGGGCAGCTCTCATCCTAA
U2 snRNA	CATCGCTTCTCGGCCTTTTG	TGGAGGTACTGCAATACCAGG
S14	GGCAGACCGAGATGAATCCTC	CAGGTCCAGGGGTCTTGGTCC
GAPDH	CAGGGGGGAGCCAAAAGGGTCA	TGGGTGGCAGTGATGGCATGGA
HuR	CCAAAUCUUUGCAUAGGUATT	UACCUAUGCAAAGAUUUGGTTA
miR-3121-3p	TAAATAGAGTAGGCAAAGGACA
miR-663a	AGGCGGGGCGCCGCGGGACCGC
miR-4529-3p	ATTGGACTGCTGATGGCCCGT
miR-505-3p	CGTCAACACTTGCTGGTTTCCT
miR-5002-5p	AATTTGGTTTCTGAGGCACTTAGT
miR-934	TGTCTACTACTGGAGACACTGG
miR-1263	ATGGTACCCTGGCATTACTGAGT
miR-5009-5p	TTGGACTTTTTCAGATTTGGGGAT
miR-2115-5p	AGCTTCCATGACTCCTGATGGA
miR-377-3p	ATCACACAAAGGCAACTTTTGT
miR-3144-3p	ATATACCTGTTCGGTCTCTTTA
miR-548av-5p	AAAAGTACTTGCGGATTT
miR-4426	GAAGATGGACGTACTTT
miR-224-5p	CAAGTCACTAGTGGTTCCGTT
miR-548k	AAAAGTACTTGCGGATTTTGCT
U6	TCGTCCCGTAGACAAAATGG

Table 2 Associations between TNFRSF10A-AS1 levels and clinicopathological features in colorectal cancer patients

Characteristics	Case	TNFRSF10A-AS1 expression		P value
		Low	High
Age (yr)				0.751
< 60	18	10	8
≥ 60	22	10	12
Gender				0.751
Male	22	12	10
Female	18	8	10
Tumor location				1.000
Colon	19	9	10
Rectum	21	11	10
Tumor size (cm)				0.001a
≥ 5	21	5	16
< 5	19	15	4
T grade				0.014a
T1 + T2	12	10	2
T3 + T4	28	10	18
TNM stage				1.000
I-II	30	15	15
III-IV	10	5	5
Histological grade				0.661
Low	6	2	4
Middle-High	34	18	16

^aP < 0.05.

Table 3 Associations between miR-3121-3p levels and clinicopathological features in colorectal cancer patients

Characteristics	Case	miR-3121-3p expression		P value
		Low	High
Age (yr)				0.751
< 60	18	10	8
≥ 60	22	10	12
Gender				0.751
Male	22	12	10
Female	18	8	10
Tumor location				1.000
Colon	19	10	9
Rectum	21	10	11
Tumor size (cm)				0.010a
≥ 5	21	15	6
< 5	19	5	14
T grade				0.082
T1 + T2	12	9	3
T3 + T4	28	11	17
TNM stage				0.716
I-II	30	14	16
III-IV	10	6	4
Histological grade				1.000
Low	6	3	3
Middle-High	34	17	17

^aP < 0.05.