Jianpi Qingchang Bushen decoction improves inflammatory response and metabolic bone disorder in inflammatory bowel disease-induced bone loss

Table 1 Calculation of disease activity index score

Score	Weight loss	Stool consistency	Bleeding
0	Normal	Normal (well-formed pellets)	Not observed
1	1%-5%	Normal (well-formed pellets)	Not observed
2	6%-10%	Loose (pasty stools that do not stick to the anus)	Occult
3	11%-15%	Loose (pasty stools that do not stick to the anus)	Occult
4	> 15%	Diarrhea (liquid stools that stick to the anus)	Gross bleeding

Table 2 List of primers used in this study

Gene	Primers(5’-3’)	Primer length
NF-κB	Forward GTGGAGGCATGTTCGGTAGTG	195
	Reverse TCTTGGCACAATCTTTAGGGC
TNF-α	Forward CCCTCCAGAAAAGACACCATG	183
	Reverse CACCCCGAAGTTCAGTAGACAG
IL-1β	Forward GCTTCAGGCAGGCAGTATCA	196
	Reverse TGCAGTTGTCTAATGGGAACG
IL-6	Forward AAATGATGGATGCTACCAAACTG	137
	Reverse CTCTGGCTTTGTCTTTCTTGTTATC
IL-8	Forward TAGTGTTGAGCATGAAAAGCCTC	152
	Reverse TGACTTCACTGGAGTCCCGTAG

NF-κB: Nuclear factor-kappaB; TNF-α: Tumor necrosis factor-α; IL: Interleukin.