Tissue microarray-chip featuring computerized immunophenotypical characterization more accurately subtypes ampullary adenocarcinoma than routine histology

Table 1 Categories used to classify ampullary adenocarcinomas

	Pure histological classification	Basal histological classification	Molecular and histological classification
AACs	5 Categories	4 Categories	3 Categories
Tubular	Pure INT	Pure INT	INT
	Mixed INT-predominant	Mixed
	Mixed PB-predominant		PB
	Pure PB	Pure PB
Non-tubular	Other	Other	Other

AACs: Ampullary adenocarcinomas; INT: Intestinal; PB: Pancreato-Biliary.

Table 2 Immunophenotypical characterization of ampullary adenocarcinomas

Ref.	Markers							Number
	CK7	CK17	CK20	CDX2	MUC1	MUC2
Chang et al[11], 2013				X	X			2
Kumari et al[22], 2013	X	X	X	X	X	X		6
Ang et al[18], 2014			X	X	X	X		4
Fernández Moro et al[16], 2016				X	X			2
Reid et al[15], 2016								0
Liu et al[24], 2019	X	X	X	X	X	X		6
Moekotte et al[31], 2018			X	X	X	X		4
Al Abbas et al[30], 2019			X	X	X	X		4
Abraham et al[17], 2020	X		X					2
Total	33%	22%	67%	78%	78%	56%	Mean	3.33

Table 3 Patient characteristics

Patient characteristics	n
Age, mean ± SD (yr)	72.9 ± 8.1
M/F	11/10
BMI, mean ± SD (kg/m2)	22.9 ± 3.9
ASA I, n (%)	3 (14.2)
ASA II, n (%)	6 (28.6)
ASA III, n (%)	11 (52.4)
ASA IV, n (%)	1 (4.8)
Comorbidity, n (%)	16 (76.1)
Cardiovascular disease, n (%)	6 (28.6)
COPD, n (%)	6 (28.6)
Hypertension, n (%)	10 (47.6)
Diabetes mellitus, n (%)	3 (14.2)
Symptomatic, n (%)	12 (57.1)
Jaundice, n (%)	7 (58.3)
Pain, n (%)	3 (25.1)
Nausea or Vomiting, n (%)	1 (8.3)
Loss of weight, n (%)	1 (8.3)
Placement of PTBD or biliary endoprothesis, n (%)	4 (33.3)
CA 19.9, mean ± SD (U/mL)	37.1 ± 53.2
Neoadjuvant therapy, n (%)	0 (0)

ASA: American Society of Anesthesiologists; COPD: Chronic obstructive pulmonary disease; PTBD: Percutaneous transhepatic biliary drainage.

Table 4 Post-operative data

LOS, mean ± SD (d)	15.4 ± 6.2
Overall complications, n (%)	7 (33.3)
Clavien-Dindo > III, n (%)	3 (14.2)
Reoperation, n (%)	2 (9.5)
30-d mortality, n (%)	0 (0)

LOS: Length of hospital stay.