Interleukin-6 compared to the other Th17/Treg related cytokines in inflammatory bowel disease and colorectal cancer

doi:10.3748/wjg.v26.i16.1912

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World Journal of Gastroenterology

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World J Gastroenterol. Apr 28, 2020; 26(16): 1912-1925
Published online Apr 28, 2020. doi: 10.3748/wjg.v26.i16.1912

Table 1 Local gene expression of pro and anti-inflammatory cytokines in paired inflamed vs non-inflamed tissues of inflammatory bowel disease patients and paired tumoral vs adjacent non-tumoral mucosa of colorectal cancer patients

Genes	IBD			CRC			P value IBD vs CRC
	dCt ± SD		RQ (min-max)	dCt ± SD		RQ (min-max)
	Inflamed tissue	Non-inflamed tissue	RQ (min-max)	Tumoral tissue	Non-tumoral tissue	RQ (min-max)
Foxp3	14.94 ± 2.65	15.81 ± 2.59	1.82 (0.11-74.85)	18.62 ± 5.5	21.30 ± 4.3	6.42 (0.47-97.2)	0.025
TGFB1	9.52 ± 4.13	8.97 ± 4.4	0.68 (0.03-2.27)	17.27 ± 2.9	18.63 ± 1.9	2.37 (0.211-93.7)	0.007
IL-10	16.87 ± 1.76	17.7 ± 1.5	1.78 (0.22-28.5)	16.43 ± 5.5	19.17 ± 4.4	6.66 (0.43-202.3)	0.023
IL-6	17.13 ± 3.05	19.53 ± 2.7	5.28 (0.27-61.6)	19.11 ± 3.8	21.07 ± 2.5	3.88 (0.03-512.4)	0.784
IL-23A	18.0 ± 2.09	18.1 ± 2.8	1.07 (0.07-6.61)	12.56 ± 2.1	17.55 ± 2.5	28.5 (2.04-219.5)	0.0003
IL-17A	18.9 ± 2.65	20.3 ± 2.9	2.70 (0.07-20.5)	24.7 ± 4.3	26.9 ± 3.0	4.69 (0.034-494.9)	0.574

The results are presented as dCt ± SD and RQ (min-max). IBD: Inflammatory bowel disease; CRC: Colorectal cancer; dCt: Normalized to the endogenous control; RQ: Relative quantity of mRNA levels.

Table 2 The local gene expression in inflammatory bowel disease patients compared to that in early colorectal cancer cases

Genes	IBD			Early stages CRC (1 + 2)			P value IBD vs Early CRC
	dCt ± SD		RQ (min-max)	dCt ± SD		RQ (min-max)
	Inflamed tissue	Non-inflamed tissue	RQ (min-max)	Tumoral tissue	Non-tumoral tissue	RQ (min-max)
Foxp3	14.94 ± 2.65	15.81 ± 2.59	1.82 (0.11-74.85)	16.98 ± 5.8	20.41 ± 4.5	10.74 (0.615-97.2)	0.023
TGFB1	9.52 ± 4.13	8.97 ± 4.4	0.68 (0.03-2.27)	16.99 ± 2.4	18.83 ± 2.3	3.59 (0.222-93.7)	0.057
IL-10	16.87 ± 1.76	17.7 ± 1.5	1.78 (0.22-28.5)	16.17 ± 5	19.25 ± 4.1	8.44 (1.253-202.3)	0.019
IL-6	17.13 ± 3.05	19.53 ± 2.7	5.28 (0.27-61.6)	18.12 ± 4.7	20.59 ± 1.9	5.55 (0.03-512.4)	0.97
IL-23A	18.0 ± 2.09	18.1 ± 2.8	1.07 (0.07-6.61)	11.69 ± 1.9	17.65 ± 2.9	61.93 (2.04-219.5)	0.001
IL-17A	18.9 ± 2.65	20.3 ± 2.9	2.70 (0.07-20.5)	22.42 ± 3.6	27.17 ± 2.9	26.83 (2.73-494.9)	0.029

The results are presented as dCt ± SD and RQ (min-max). IBD: Inflammatory bowel disease; CRC: Colorectal cancer; dCt: Normalized to the endogenous control; RQ: Relative quantity of mRNA levels.

Table 3 The local gene expression in inflammatory bowel disease patients compared to that in advanced colorectal cancer cases

Genes	IBD			Advanced stages CRC (3 + 4)			P value IBD vs Advanced CRC
	dCt ± SD		RQ (min-max)	dCt ± SD		RQ (min-max)
	Inflamed tissue	Non-inflamed tissue	RQ (min-max)	Tumoral tissue	Non-tumoral tissue	RQ (min-max)
Foxp3	14.94 ± 2.65	15.81 ± 2.59	1.82 (0.11-74.85)	19.71 ± 5.2	21.89 ± 4.2	4.55 (0.45-45.1)	0.101
TGFB1	9.52 ± 4.13	8.97 ± 4.4	0.68 (0.03-2.27)	17.46 ± 3.3	18.5 ± 1.6	2.05 (0.211-14.56)	0.176
IL-10	16.87 ± 1.76	17.7 ± 1.5	1.78 (0.22-28.5)	16.69 ± 6.2	19.09 ± 5	5.26 (0.43-158.8)	0.113
IL-6	17.13 ± 3.05	19.53 ± 2.7	5.28 (0.27-61.6)	19.78 ± 3.1	21.39 ± 2.8	3.05 (0.025-434.7)	0.608
IL-23A	18.0 ± 2.09	18.1 ± 2.8	1.07 (0.07-6.61)	13.64 ± 2.1	17.44 ± 2.4	13.96 (3.93-36.28)	0.012
IL-17A	18.9 ± 2.65	20.3 ± 2.9	2.70 (0.07-20.5)	26.62 ± 3.9	26.73 ± 3.2	1.07 (0.034-89.26)	0.350

The results are presented as dCt ± SD and RQ (min-max). IBD: Inflammatory bowel disease; CRC: Colorectal cancer; dCt: Normalized to the endogenous control; RQ: Relative quantity of mRNA levels.

Table 4 Serum levels of pro and anti-inflammatory cytokines in inflammatory bowel disease and colorectal cancer patients

Cytokines	IBD patients	CRC patients	P value (U-test)
TGFB1 (ng/mL)	11.19 (9.36-20.99)	22.57 (18.08-29.65)	0.0008
IL-10 (pg/mL)	0.4 (0.0-2.8)	8.29 (6.75-15.12)	0.000001
IL-6 (pg/mL)	0.0 (0.0-2.7)	3.21 (2.03-4.19)	0.012
IL-23A (pg/mL)	0.7 (0.0-3.7)	27.55 (25.35-33.0)	0.000001
IL-17A (pg/mL)	0.0 (0.0-0.26)	2.85 (0.0-9.0)	0.034

The results are presented as median (IQR: 25%-75% percentile). IBD: Inflammatory bowel disease; CRC: Colorectal cancer.

Table 5 Serum levels of anti and pro-inflammatory cytokines in inflammatory bowel disease patients compared to early and advanced colorectal cancer cases

Cytokines	IBD	Early CRC	P value (U-test), IBD vs early CRC	Advanced CRC	P value (U-test), IBD vs advanced CRC
TGFB1 (ng/mL)	11.19 (9.36-20.99)	22.74 (18.17-27.98)	0.001344	21.51 (15.5-31.05)	0.0031
IL-10 (pg/mL)	0.4 (0.0-2.8)	7.73 (6.0-12.71)	0.000006	8.84 (7.2-19.81)	0.000002
IL-6 (pg/mL)	0.0 (0.0-2.7)	3.06 (2.03-4.19)	0.0153	4.19 (2.15-8.4)	0.044
IL-23A (pg/mL)	0.7 (0.0-3.7)	25.7 (24.6-32.5)	0.000024	30.5 (26.3-33.5)	0.000009
IL-17A (pg/mL)	0.0 (0.0-0.26)	3.62 (0.0-8.23)	0.0086	1.2 (0.0-12.08)	0.178

The results are presented as median (IQR, 25%-75% percentile). IBD: Inflammatory bowel disease; CRC: Colorectal cancer.

Citation: Velikova TV, Miteva L, Stanilov N, Spassova Z, Stanilova SA. Interleukin-6 compared to the other Th17/Treg related cytokines in inflammatory bowel disease and colorectal cancer. World J Gastroenterol 2020; 26(16): 1912-1925
URL: https://www.wjgnet.com/1007-9327/full/v26/i16/1912.htm
DOI: https://dx.doi.org/10.3748/wjg.v26.i16.1912