Velikova TV, Miteva L, Stanilov N, Spassova Z, Stanilova SA. Interleukin-6 compared to the other Th17/Treg related cytokines in inflammatory bowel disease and colorectal cancer. World J Gastroenterol 2020; 26(16): 1912-1925 [PMID: 32390702 DOI: 10.3748/wjg.v26.i16.1912]
Corresponding Author of This Article
Tsvetelina Veselinova Velikova, MD, PhD, Assistant professor, Clinical Immunology, University Hospital Lozenetz, Kozyak 1 Street, Sofia 1407, Bulgaria. tsvelikova@medfac.mu-sofia.bg
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
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World J Gastroenterol. Apr 28, 2020; 26(16): 1912-1925 Published online Apr 28, 2020. doi: 10.3748/wjg.v26.i16.1912
Table 1 Local gene expression of pro and anti-inflammatory cytokines in paired inflamed vs non-inflamed tissues of inflammatory bowel disease patients and paired tumoral vs adjacent non-tumoral mucosa of colorectal cancer patients
Genes
IBD
CRC
P value IBD vs CRC
dCt ± SD
RQ (min-max)
dCt ± SD
RQ (min-max)
Inflamed tissue
Non-inflamed tissue
Tumoral tissue
Non-tumoral tissue
Foxp3
14.94 ± 2.65
15.81 ± 2.59
1.82 (0.11-74.85)
18.62 ± 5.5
21.30 ± 4.3
6.42 (0.47-97.2)
0.025
TGFB1
9.52 ± 4.13
8.97 ± 4.4
0.68 (0.03-2.27)
17.27 ± 2.9
18.63 ± 1.9
2.37 (0.211-93.7)
0.007
IL-10
16.87 ± 1.76
17.7 ± 1.5
1.78 (0.22-28.5)
16.43 ± 5.5
19.17 ± 4.4
6.66 (0.43-202.3)
0.023
IL-6
17.13 ± 3.05
19.53 ± 2.7
5.28 (0.27-61.6)
19.11 ± 3.8
21.07 ± 2.5
3.88 (0.03-512.4)
0.784
IL-23A
18.0 ± 2.09
18.1 ± 2.8
1.07 (0.07-6.61)
12.56 ± 2.1
17.55 ± 2.5
28.5 (2.04-219.5)
0.0003
IL-17A
18.9 ± 2.65
20.3 ± 2.9
2.70 (0.07-20.5)
24.7 ± 4.3
26.9 ± 3.0
4.69 (0.034-494.9)
0.574
Table 2 The local gene expression in inflammatory bowel disease patients compared to that in early colorectal cancer cases
Genes
IBD
Early stages CRC (1 + 2)
P value IBD vs Early CRC
dCt ± SD
RQ (min-max)
dCt ± SD
RQ (min-max)
Inflamed tissue
Non-inflamed tissue
Tumoral tissue
Non-tumoral tissue
Foxp3
14.94 ± 2.65
15.81 ± 2.59
1.82 (0.11-74.85)
16.98 ± 5.8
20.41 ± 4.5
10.74 (0.615-97.2)
0.023
TGFB1
9.52 ± 4.13
8.97 ± 4.4
0.68 (0.03-2.27)
16.99 ± 2.4
18.83 ± 2.3
3.59 (0.222-93.7)
0.057
IL-10
16.87 ± 1.76
17.7 ± 1.5
1.78 (0.22-28.5)
16.17 ± 5
19.25 ± 4.1
8.44 (1.253-202.3)
0.019
IL-6
17.13 ± 3.05
19.53 ± 2.7
5.28 (0.27-61.6)
18.12 ± 4.7
20.59 ± 1.9
5.55 (0.03-512.4)
0.97
IL-23A
18.0 ± 2.09
18.1 ± 2.8
1.07 (0.07-6.61)
11.69 ± 1.9
17.65 ± 2.9
61.93 (2.04-219.5)
0.001
IL-17A
18.9 ± 2.65
20.3 ± 2.9
2.70 (0.07-20.5)
22.42 ± 3.6
27.17 ± 2.9
26.83 (2.73-494.9)
0.029
Table 3 The local gene expression in inflammatory bowel disease patients compared to that in advanced colorectal cancer cases
Genes
IBD
Advanced stages CRC (3 + 4)
P value IBD vs Advanced CRC
dCt ± SD
RQ (min-max)
dCt ± SD
RQ (min-max)
Inflamed tissue
Non-inflamed tissue
Tumoral tissue
Non-tumoral tissue
Foxp3
14.94 ± 2.65
15.81 ± 2.59
1.82 (0.11-74.85)
19.71 ± 5.2
21.89 ± 4.2
4.55 (0.45-45.1)
0.101
TGFB1
9.52 ± 4.13
8.97 ± 4.4
0.68 (0.03-2.27)
17.46 ± 3.3
18.5 ± 1.6
2.05 (0.211-14.56)
0.176
IL-10
16.87 ± 1.76
17.7 ± 1.5
1.78 (0.22-28.5)
16.69 ± 6.2
19.09 ± 5
5.26 (0.43-158.8)
0.113
IL-6
17.13 ± 3.05
19.53 ± 2.7
5.28 (0.27-61.6)
19.78 ± 3.1
21.39 ± 2.8
3.05 (0.025-434.7)
0.608
IL-23A
18.0 ± 2.09
18.1 ± 2.8
1.07 (0.07-6.61)
13.64 ± 2.1
17.44 ± 2.4
13.96 (3.93-36.28)
0.012
IL-17A
18.9 ± 2.65
20.3 ± 2.9
2.70 (0.07-20.5)
26.62 ± 3.9
26.73 ± 3.2
1.07 (0.034-89.26)
0.350
Table 4 Serum levels of pro and anti-inflammatory cytokines in inflammatory bowel disease and colorectal cancer patients
Cytokines
IBD patients
CRC patients
P value (U-test)
TGFB1 (ng/mL)
11.19 (9.36-20.99)
22.57 (18.08-29.65)
0.0008
IL-10 (pg/mL)
0.4 (0.0-2.8)
8.29 (6.75-15.12)
0.000001
IL-6 (pg/mL)
0.0 (0.0-2.7)
3.21 (2.03-4.19)
0.012
IL-23A (pg/mL)
0.7 (0.0-3.7)
27.55 (25.35-33.0)
0.000001
IL-17A (pg/mL)
0.0 (0.0-0.26)
2.85 (0.0-9.0)
0.034
Table 5 Serum levels of anti and pro-inflammatory cytokines in inflammatory bowel disease patients compared to early and advanced colorectal cancer cases
Cytokines
IBD
Early CRC
P value (U-test), IBD vs early CRC
Advanced CRC
P value (U-test), IBD vs advanced CRC
TGFB1 (ng/mL)
11.19 (9.36-20.99)
22.74 (18.17-27.98)
0.001344
21.51 (15.5-31.05)
0.0031
IL-10 (pg/mL)
0.4 (0.0-2.8)
7.73 (6.0-12.71)
0.000006
8.84 (7.2-19.81)
0.000002
IL-6 (pg/mL)
0.0 (0.0-2.7)
3.06 (2.03-4.19)
0.0153
4.19 (2.15-8.4)
0.044
IL-23A (pg/mL)
0.7 (0.0-3.7)
25.7 (24.6-32.5)
0.000024
30.5 (26.3-33.5)
0.000009
IL-17A (pg/mL)
0.0 (0.0-0.26)
3.62 (0.0-8.23)
0.0086
1.2 (0.0-12.08)
0.178
Citation: Velikova TV, Miteva L, Stanilov N, Spassova Z, Stanilova SA. Interleukin-6 compared to the other Th17/Treg related cytokines in inflammatory bowel disease and colorectal cancer. World J Gastroenterol 2020; 26(16): 1912-1925