Ubiquitin-conjugating enzyme E2T knockdown suppresses hepatocellular tumorigenesis via inducing cell cycle arrest and apoptosis

Table 1 The primers of target genes

Gene	Primer sequence
UBE2T	Forward	TTGATTCTGCTGGAAGGATTTG
	Reverse	CAGTTGCGATGTTGAGGGAT
PTGS2	Forward	CTCCTGTGCCTGATGATTGC
	Reverse	CAGCCCGTTGGTGAAAGC
IL1B	Forward	TCCTGTTGTCTACACCAATGCCCA
	Reverse	GAACCAAATGTGGCCGTGGTTTCT
RAC2	Forward	GAAGCATCTACCCGTTCACTC
	Reverse	AGTTGTGGCAGCAACCATCT
ITGA2	Forward	CCTACAATGTTGGTCTCCCAGA
	Reverse	AGTAACCAGTTGCCTTTTGGATT
IL1A	Forward	AGATGCCTGAGATACCCAAAACC
	Reverse	CCAAGCACACCCAGTAGTCT
FOSL1	Forward	ACCCTCCCTAACTCCTTTCA
	Reverse	CTGGAGTTGGATGTGGGATA
SOD2	Forward	TGGACAAACCTCAGCCCT
	Reverse	TGAAACCAAGCCAACCC
BMP6	Forward	TCAACTTATCCCAGATTCCTGA
	Reverse	CCATACTACACGGGTGTCCAA
BCL2L1	Forward	CTGAATCGGAGATGGAGACC
	Reverse	GAGCTGGGATGTCAGGTCA
GSTO1	Forward	GCATACCCAGGGAAGAAGCT
	Reverse	AGAATTGCCACCAAAGAAGG
ITGA5	Forward	GGCTTCAACTTAGACGCGGAG
	Reverse	TGGCTGGTATTAGCCTTGGGT
ENC1	Forward	ACATGGTAGTGCAACTCTTGTC
	Reverse	TTCAGGTCATAGCTGATCCAGT
IL1RAP	Forward	CAAAGTGATGCCTCAGAACG
	Reverse	CTGCCTAGTCCAATACCAGATC
MET	Forward	AGTCATAGGAAGAGGGCATT
	Reverse	CTTCACTTCGCAGGCAGA
SOCS3	Forward	GCCTCAAGACCTTCAGCTCCAA
	Reverse	CTCCAGTAGAAGCCGCTCTCCT
PRKAR1A	Forward	GTTTTCGGTCTCCTTTATCGC
	Reverse	ACTGGTTGCCCATTCATTGTT
RPL31	Forward	CTCGGGCACTCAAAGAGATTC
	Reverse	CGGATTCGGTATGGCACATTC
PPARGC1A	Forward	TCTGAGTCTGTATGGAGTGACAT
	Reverse	CCAAGTCGTTCACATCTAGTTCA
RPL13A	Forward	GCCATCGTGGCTAAACAGGTA
	Reverse	GTTGGTGTTCATCCGCTTGC
NQO1	Forward	AGGCAGTGCTTTCCATCA
	Reverse	CAGGCGTTTCTTCCATCC
ABCC1	Forward	GTCGGGGCATATTCCTGGC
	Reverse	CTGAAGACTGAACTCCCTTCCT
RPL32	Forward	ACCCAGAGGCATTGACAACA
	Reverse	GAGCGATCTCGGCACAGTAA
GCLM	Forward	ATCAGTGGGCACAGGTAAA
	Reverse	CAGAAAGCAGTTCTTTTGGA
FYN	Forward	GGATGCCAAGGCTTACCGAT
	Reverse	GGGCTCCTCAGACACCACTG
HMOX1	Forward	AAGACTGCGTTCCTGCTCAAC
	Reverse	AAAGCCCTACAGCAACTGTCG
SCNN1A	Forward	AGTTCCACCGCTCCTACCGA
	Reverse	GTCCGAGTTGAGGTTGATGTTG
GSTA4	Forward	ACTATCCCAACGGAAGAGGC
	Reverse	CAGGTGGTTACCATCCTGCA
MAP2K6	Forward	GAAGCATTTGAACAACCTCAGAC
	Reverse	CCTGGCTATTTACTGTGGCTC
PIK3C2B	Forward	TTCCTCCACTGTAGACTTGCTT
	Reverse	AGCCGAATGTCAATGTCAAACT
GAPDH	Forward	TGACTTCAACAGCGACACCCA
	Reverse	CACCCTGTTGCTGTAGCCAAA

Table 2 Relationship between ubiquitin-conjugating enzyme E2T expression and clinicopathological parameters by The Cancer Genome Atlas

		Expression of UBE2T		Total	P value
		Low	High
Grade					0.000
	G1/2	137	95	232
	G3/4	47	87	134
Total		184	182	366
T stage					0.001
	T1	108	73	181
	T2	38	56	94
	T3/4	38	55	93
Total		184	184	368
Pathological stage					0.002
	I	101	70	171
	II	36	50	86
	III/IV	37	53	90
Total		174	173	347

UBE2T: Ubiquitin-conjugating enzyme E2T.