MiR-32-5p aggravates intestinal epithelial cell injury in pediatric enteritis induced by Helicobacter pylori

doi:10.3748/wjg.v25.i41.6222

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Nov 7, 2019; 25(41): 6222-6237
Published online Nov 7, 2019. doi: 10.3748/wjg.v25.i41.6222

Table 1 Sequences of primers, siRNAs, microRNA mimic, and microRNA inhibitor used in this study

Gene	Sequence (5’-3’)
miR-32-5p	Forward: CGGTATTGCACATTACTAAGTTGCA
miR-32-5p	Reverse: CTCGCTTCGGCAGCACA
U6	Forward: AGGGGCCATCCACAGTCTTC
U6	Reverse: AACGCTTCACGAATTTGCGT
GAPDH	Forward: AAAAGGGCCCTGACAACTCTT
GAPDH	Reverse: ACCCTGTTGCTGTAGCCAAA
SMAD6	Forward: TTGTCATTTAGGGACCCTCAGC
SMAD6	Reverse: TTGGCAGGAAATGCAGGTTTG
TNF-α	Forward: AAGATAGGGTGTCTGGCACA
TNF-α	Reverse: CCCTGAGGTGTCTGGTTTTCT
IL-6	Forward: AGCAGGCACCCCAGTTAAT
IL-6	Reverse: AATCCTTTGCAGTGGAGGGA
SMAD6 siRNA	Forward: CCACAUUGUCUUACACUGAAACGGA
SMAD6 siRNA	Reverse: UCCGUUUCAGUGUAAGACAAUGUGG
si-control	AAUUCUCCGAACGUGUCACGU
MiR-32-5p mimic	Forward: UAUUGCACAUUACUAAGUUGCA
MiR-32-5p mimic	Reverse: CAACUUAGUAAUGUGCAAUAUU
Mimic NC	UUCUCCGAACGUGUCACUGUU
MiR-32-5p inhibitor	UGCAACUUAGUAAUGUGCAAUA
Inhibitor NC	CAGUACUUUUGUGUAGUACAA

U6: U6 small nuclear RNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; SMAD6: SMAD family member 6; NC: Negative control.

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Citation: Feng J, Guo J, Wang JP, Chai BF. MiR-32-5p aggravates intestinal epithelial cell injury in pediatric enteritis induced by Helicobacter pylori. World J Gastroenterol 2019; 25(41): 6222-6237
URL: https://www.wjgnet.com/1007-9327/full/v25/i41/6222.htm
DOI: https://dx.doi.org/10.3748/wjg.v25.i41.6222