Long non-coding RNA highly up-regulated in liver cancer promotes exosome secretion

Table 1 Relationship between expression of long non-coding RNA highly up-regulated in liver cancer and clinicopathological factors in patients with hepatocellular carcinoma

Clinicopathological factor	Number of patients (n = 30)	HULC in serum exosomes		χ2	P-value	HULC in HCC tissue		χ2	P-value
		High	Low			High	Low
Age (yr)				0.391	0.532			0.382	0.497
≤ 55	18	11	7			12	8
> 55	12	7	5			5	5
sex				0.370	0.492			0.365	0.592
Female	21	11	7			13	8
Male	9	6	6			4	5
Tumor size (cm)				2.323	0.124			2.225	0.126
≤ 5	10	4	6			5	5
> 5	20	11	9			10	10
Tumor differentiation				2.224	0.115			2.356	0.098
Well/moderate	21	12	9			11	8
Poor	9	5	4			6	5
Clinical stage (TNM)				4.812	0.032			4.924	0.026
I-II	10	5	5			4	6
III-IV	20	11	9			12	8

HULC: Highly upregulated in liver cancer; HCC: Hepatocellular carcinoma; TNM: Tumor-node-metastasis.

Table 2 Primers used for quantitative PCR

Gene	Primer sequence
HULC	Forward: 5’-GCAAGCCAGGAAGAGTCGTC-3'
	Reverse: 5’-GCTGTGCTTAGTTTATTGCCAGG-3'
GAPDH	Forward: 5'-GTCAACGGATTTGGTCTGTATT-3'
	Reverse: 5'-AGTCTTCTGGGTGGCAGTGAT-3'
miRNA-372-3p	Forward: 5'-CAACAGAAGGCTCGAGCAACCTGCGGAGAAGATAC-3'
	Reverse: 5'-TTCTGATCAGGATCCCATTACAGCCAGACGCTGTAAG-3'
U6	Forward: 5'-GCGCGTCGTGAAGCGTTC-3'
	Reverse: 5'-GTGCAGGGTCCGAGGT-3'
Rab11a	Forward: 5'-ACATCAGCATATTATCGTGGAGC-3'
	Reverse: 5'-GGAACTGCCCTGAGATGACG-3'

HULC: Highly upregulated in liver cancer.