Significance of tumor-infiltrating immunocytes for predicting prognosis of hepatitis B virus-related hepatocellular carcinoma

Table 1 Baseline patient characteristics

Variable		Number (n)	Percent (%)
Age	60.1 ± 15.4	53	100
Gender
Male		31	58.5
Female		22	41.5
Race
White		33	62.3
Others		20	37.7
Grade
I		18	34.0
II		16	30.2
III		14	26.4
IV		5	9.4
Stage
I		5	9.4
II		25	47.2
III		22	41.5
IV		1	1.9

Grade: Neoplasm histologic grade; Stage: TNM stage.

Table 2 Thresholds for immunocyte fractions

Cell type	Cut-off value
B cells naive	0.107
B cells memory	0
Plasma cells	0.010
T cells CD8	0.020
T cells CD4 memory resting	0.157
T cells CD4 memory activated	0
T cells follicular helper	0.054
T cells regulatory	0.108
NK cells resting	0.002
NK cells activated	0.035
Monocytes	0.082
Macrophages M0	0.067
Macrophages M1	0.030
Macrophages M2	0.131
Dendritic cells resting	0.039
Dendritic cells activated	0.002
Mast cells resting	0.074
Mast cells activated	0
Eosinophils	0
Neutrophils	0.013

Table 3 Cox regression analysis for overall survival-related clinicopathological features

Parameter	Univariate analysis		Multivariate analysis
	HR (95%CI)	P-value	HR (95%CI)	P-value
Risk_score	2.010 (1.487-2.716)	< 0.001	2.997 (1.737-5.170)	< 0.001
Age	1.022 (0.985-1.060)	0.255	1.091 (1.024-1.162)	0.007
Gender (vs male)	1.001 (0.404-2.479)	0.999	0.727 (0.215-2.459)	0.608
Race (vs white)	1.369 (0.470-3.992)	0.565	0.905 (0.195-4.208)	0.899
Grade (vs I/II)	1.800 (0.743-4.359)	0.193	0.987 (0.327-2.975)	0.981
Stage (vs I/II)	2.161 (0.865-5.400)	0.099	10.182 (2.251-46.058)	0.003

Grade: Neoplasm histologic grade; Stage: TNM stage; HR: Hazard ratio; CI: Confidence interval.