Effect of mild moxibustion on intestinal microbiota and NLRP6 inflammasome signaling in rats with post-inflammatory irritable bowel syndrome

Table 1 Primers used for amplification of selective intestinal microbiota

Microorganism		Base sequence
Lactobacillus	Primer F	5'-ACGGGAGGCAGCAGTAGGGA-3'
	Primer R	5'-AGCCGTGACTTTCTGGTTGATT-3'
Bifidobacterium	Primer F	5'-GATTCTGGCTCAGGATGAACGC-3'
	Primer R	5'-CTGATAGGACGCGACCCCAT-3'
Faecalibacterium_prausnitzii	Primer F	5'-AGATGGCCTCGCGTCCGA-3'
	Primer R	5'-CCGAAGAACCTTCTTCCTCC-3'
Escherichia_coli	Primer F	5'-CATGCCGCGTGTATGAAGAA-3'
	Primer R	5'-CGGGTAACGTCAATGAGCAAA-3'

Table 2 Primers used for amplification of NLRP6, ASC, Caspase-1, and GAPH mRNA

mRNA		Base sequence
NLRP6	Primer A	5'-AAGGTGAAGGAGAGGAATG-3'
	Primer B	5'-GAAGAGCCGATTGAAAGTG-3'
ASC	Primer A	5'-TGCTGGATGCTCTGTATGG-3'
	Primer B	5'-CAAGTAGGGCTGTGTTTGC-3'
Caspase-1	Primer A	5'-GACAAGCCCAAGGTTATC-3'
	Primer B	5'-GGCCTTCTTAATGCCATC-3'
IL-1β	Primer A	5'-AAGGTGAAGGAGAGGAAT-3'
	Primer B	5'-GAAGAGCCGATTGAAAGTG-3'
IL-18	Primer A	5'-TGCTGGATGCTCTGTATGG-3'
	Primer B	5'-CAAGTAGGGCTGTGTTTGC-3'
GAPDH	Primer A	5'-GTCGGTGTGAACGGATTTG-3'
	Primer B	5'-TCCCATTCTCAGCCTTGAC-3'

Table 3 Correlations between selective intestinal microbes and mRNA and protein expression of NLRP6, ASC, and Caspase-1

	Lactobacillus		Bifidobacterium		Faecalibacterium_prau-snitzii		Escherichia_coli
	r	P	r	P	r	P	r	P
NLRP6 mRNA	0.550	0.000	0.542	0.000	0.624	0.000	-0.495	0.000
ASC mRNA	-0.641	0.000	-0.633	0.000	-0.747	0.000	0.432	0.005
Caspase-1 mRNA	-0.656	0.000	-0.688	0.000	-0.737	0.000	0.716	0.000
NLRP6 protein	0.639	0.000	0.750	0.000	0.920	0.000	-0.626	0.000
ASC protein	-0.607	0.000	-0.709	0.000	-0.797	0.000	0.606	0.000
Caspase-1 protein	-0.654	0.000	-0.669	0.000	-0.749	0.000	0.501	0.001