MicroRNA-15a - cell division cycle 42 signaling pathway in pathogenesis of pediatric inflammatory bowel disease

Table 1 Primer sequences for quantitative reverse transcription polymerase chain reaction

Primer	Primer sequences (5' → 3')
miR-15a	F: 5’- ACACTCCAGCTGGGTAGCAGCACATAATGG-3’
	R: 10 × miscript universal primer (QIAGEN)
RNU6A	F: 5’- CTCGCTTCGGCAGCACA-3’
	R: 5’- AACGCTTCACGAATTTGCGT -3’
Cdc42	F: 5’- TTATGACAGATTACGACCGC -3’
	R: 5’- CCAACAAGCAAGAAAGGAGT -3’
ZO-1	F: 5’- AACAAGCCAGCAGAGACCTC -3’
	R: 5’- CTTCATACATGGGGACGCGA -3’
E-cadherin	F: 5’- TCCAGTGAACAACGATGGCA -3’
	R: 5’- AATGTACTGCTGCTTGGCCT -3’
GAPDH	F: 5’- CCTCCTCACAGTTGCCATGT -3’
	R: 5’- CTGGTTGAGCACAGGGTACT -3’

miR-15a: MicroRNA-15a; Cdc42: Cell division cycle 42; ZO-1: Zona occludens-1.

Table 2 Demographic characteristics of all patients

	Control	CD	UC
n	21	26	7
Male	12	14a	4a
Female	9	12a	3a
Age, yr (mean ± SD)	9.773 ± 0.7755	10.84 ± 0.6690a	9.743 ± 1.209a

^aP > 0.05 vs control. CD: Crohn’s disease; UC: Ulcerative colitis.

Table 3 Clinical characteristics of matched inflammatory bowel disease patients and controls

Matched group	Patient no.	Group	Sex	Age, yr	Disease	PCDAI/PUCAI
1	1	Control	M	3	JP
	2	Control	M	3	JP
	3	CD AC	M	3	CD	52.5
	4	CD AC	M	2.5	CD	25
2	5	Control	F	7	JP
	6	Control	F	7	JP
	7	CD RE	M	8	CD	0
	8	CD RE	F	7	CD	2.5
	9	UC	F	7	UC	30
3	10	Control	M	8	JP
	11	Control	M	9	JP
	12	CD AC	M	7.5	CD	27.5
	13	UC	M	8	UC	20
	14	UC	M	8	UC	20
4	15	Control	F	9	JP
	16	CD AC	F		CD	40
5	17	Control	M	10	JP
	18	Control	M	10	JP
	19	Control	M	10	JP
	20	CD AC	M	9.6	CD	20
	21	CD AC	M	10	CD	30
	22	CD RE	M	10	CD	5
6	23	Control	M	11	JP
	24	Control	M	11	JP
	25	CD AC	M	11	CD	32.5
	26	CD AC	M	12.2	CD	50
	27	CD AC	M	11	CD	45
	28	CD AC	F	11	CD	15
7	29	Control	F	12	JP
	30	Control	F	12	JP
	31	Control	F	12	JP
	32	CD AC	F	12	CD	25
	33	CD AC	F	12	CD	35
	34	CD AC	F	12	CD	32.5
	35	CD AC	F	12	CD	47.5
	36	CD RE	M	13.1	CD	0
	37	UC	M	13.1	UC	35
	38	UC	F	12	UC	20
8	39	Control	F	13	JP
	40	CD AC	F	13	CD	45
	41	CD AC	F	12.8	CD	32.5
9	42	Control	F	14	JP
	43	CD AC	F	14	CD	20
	44	UC	F	14	UC	40
	45	CD RE	F	14	CD	2.5
10	46	Control	M	14	JP
	47	CD RE	M	15.1	CD	0
	48	CD AC	M	14	CD	30
	49	CD AC	M	14	CD	47.5
11	50	Control	F	16	JP
	51	CD RE	F	15.8	CD	5
12	52	Control	M	5	JP
	53	Control	M	5	JP
	54	UC	M	6.1	UC	35

M: Male; F: Female; JP: Juvenile polyps; CD: Crohn’s disease; CD AC: Crohn’s disease active; CD RE: Crohn’s disease remission; UC: Ulcerative colitis; PCDAI: Pediatric Crohn’s Disease Activity Index; PUCAI: Pediatric Ulcerative Colitis Activity Index.