Yiguanjian decoction enhances fetal liver stem/progenitor cell-mediated repair of liver cirrhosis through regulation of macrophage activation state

Table 1 Primer pairs and probes used for real-time PCR

Gene		Primer sequence	Note
α-SMA	Forward	AATGGCTCTGGGCTCTGTAA	SYBR
	Reverse	TCTCTTGCTCTGGGCTTCAT
Col1	Forward	TGACTGGAAGAGCGGAGAGT	SYBR
	Reverse	GACGGCTGAGTAGGGAACAC
TGF-β	Forward	ATTCCTGGCGTTACCTTGG	SYBR
	Reverse	AGCCCTGTATTCCGTCTCCT
TNF-α	Forward	GACGTGGAACTGGCAGAAGAG	SYBR
	Reverse	TTGGTGGTTTGTGAGTGTGAG
CD68	Forward	GGACCCACAACTGTCACTCAT	SYBR
	Reverse	AAGCCCCACTTTAGCTTTACC
CD163	Forward	TGGGATCGCCGTGACGCTTC	SYBR
	Reverse	CAGCGACTGCCTCCACCGAC
HNF4α	Forward	CGGGCCACTGGCAAACAC	SYBR
	Reverse	GTAATCCTCCAGGCTCAC
Hep	Forward	TAGCAGAGATGAGCCGTGTG	SYBR
	Reverse	GCTTTGAGGCAGGCGTATT
β-catenin	Forward	GTCTGAGGACAAGCCACAGGACTAC	SYBR
	Reverse	AATGTCCAGTCCGAGATCAGCA
Wnt 1	Forward	GGGGAGCAACCAAAGTCG	SYBR
	Reverse	TGGAGGAGGCTATGTTCACG
Wnt 3A	Forward	TCCGACTCTTGGCAGAACTT-3	SYBR
	Reverse	AATGGAATAGGTCCCGAACA
Wnt 4	Forward	GGCACTCATGAACCTTCACAACA	SYBR
	Reverse	CTTTACCTCACAGGAGCCTGACAC
Wnt 5A	Forward	GCGCTGCTGGAGTGGTAAAT	SYBR
	Reverse	AGCCAGTCCCGAGGTAAGTC
Wnt 5B	Forward	CGAGCCCTCATGAACTTACAGAAC	SYBR
	Reverse	GGAGACTCCGTGACATTTGCAG
Wnt 8A	Forward	CCTGGGAGCGGTGGAACT	SYBR
	Reverse	CCTGGTGTGGGTTGAAAACTG
Wnt 8B	Forward	AAGGCTTACCTGGTCTACTC	SYBR
	Reverse	CAGAGCTGATGGCGTGCACA
Wnt 10B	Forward	CCTCAAGCGCGGTTTCC	SYBR
	Reverse	CAGCAGCCAGCATGGAGAA
Wnt 11	Forward	TTGACCTGGAGAGAGGTACAC	SYBR
	Reverse	GTCAGGGGAGCTCTGTAGATA
Frizzled 1	Forward	GGGAATGCAGTCACCAGTACCA	SYBR
	Reverse	CCAGACCCATAGCAGGTTCCA
Frizzled 2	Forward	ACTGCAAGAGCCTAGCCATCC	SYBR
	Reverse	ATCCAGAAGCCCGACGTGA
Frizzled 3	Forward	ACACATGGCACCAGCATGAAC	SYBR
	Reverse	CCATGCGAAGGCCAAGACTAA
Frizzled 4	Forward	GACAACTTTCACGCCGCTCA	SYBR
	Reverse	TTCAGGACTGGTTCACATCGTCTC
Frizzled 5	Forward	CGAGAGCACAGCCACATTCACTA	SYBR
	Reverse	GAGCTGGCCATGCCAAAGA
Frizzled 6	Forward	CAGCAGCGTCCAACTCCAAG	SYBR
	Reverse	TGCACTCCATCAGGCCAGTC
LRP5	Forward	GACATTTACTGGCCCAATGG	SYBR
	Reverse	CTGCCCTCCACCACCTTCT
LRP6	Forward	TCTCCGGCGAATTGAAAG	SYBR
	Reverse	GAGTCTTCTAGCACGATCCTGT
GAPDH	Forward	AAGGTCATCCATGACAACTTTGGC	SYBR
	Reverse	ACAGTCTTCTGGGTGGCAGTGAT

α-SMA: Alpha-smooth muscle actin; Col(1): Collagen type I; TGF-β: Transforming growth factor beta; TNF-α: Tumor necrosis factor-alpha; HNF4α: Hepatocyte nuclear factor 4 alpha; Hep: Hep Par-1; LRP: Low-density lipoprotein receptor-related protein; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.