Moxibustion treatment modulates the gut microbiota and immune function in a dextran sulphate sodium-induced colitis rat model

Table 1 Histopathological Scores

Histopathological manifestation		score
Ulcer	No ulcer	0
	Ulcer area < 3 cm	1
	Ulcer area > 3 cm	2
Inflammation	No inflammation	0
	Mild inflammation	1
	Severe inflammation	2
Granuloma	No granuloma	0
	Granuloma	1
Lesion depth	No lesion	0
	Submucosa	1
	Muscular layer	2
	Serosa layer	3
Fibrosis	No fibrosis	0
	Mild fibrosis	1
	Severe fibrosis	2

Table 2 Primer sequences

Target gene	Forward sequence	Reverse sequence
IL-6	GCCAGAGTCATTCAGAGCAA	TTGGTCCTTAGCCACTCCTT
IL-10	CTGTCATCGATTTCTCCCCT	CAAACTCATTCATGGCCTTG
IL-12	CCCTCAAGTTCTTCGTCCGC	TTTGCATTGGACTTCGGCAG
IL-17	ATCCAGCAAGAGATCCTGGT	CAATAGAGGAAACGCAGGTG
IL-23	TGTGGTGATGGTTGTGATCC	TGTGAAGATGTCCGAGTCCA
TNF-α	GAAAGCATGATCCGAGATGT	CAGGAATGAGAAGAGGCTGA
TNFR1	TGAGACGCATTTCCAGTGTG	AGAATCCTGCGTGGCAGTTA
TNFR2	CATGTCAACGTCACCTGCAT	TCATCCTTTGGAGACCCTGA
rACTIN	ACTTCGAGCAGGAGATGGCC	CCCAGGAAGGAAGGCTGGAA