Detection of hyper-conserved regions in hepatitis B virus X gene potentially useful for gene therapy

Table 1 Main clinical and virological characteristics of the hepatitis B virus infected patients enrolled

Patient	Age	Sex	Origin	Clinical stage	HBeAg	HBV DNA(log IU/mL)	ALT (IU/L)	Genotype1
1	27	M	Sub-Saharan	Chronic hepatitis	Negative	7.8	170	E
2	31	M	Asian	Chronic hepatitis	Negative	6.6	103	C
3	51	M	Caucasian	Chronic hepatitis	Negative	6.6	262	D
4	28	F	Caucasian	Chronic hepatitis	Negative	7.9	126	D
5	47	F	Caucasian	Chronic hepatitis	Negative	6.3	170	D
6	37	F	Caucasian	Chronic hepatitis	Negative	4.5	53	D
7	37	M	Caucasian	Chronic hepatitis	Negative	4.5	33	F
8	38	M	Caucasian	Chronic hepatitis	Negative	5.0	29	D
9	46	M	Caucasian	Chronic hepatitis	Negative	5.8	88	F
10	46	F	Caucasian	Chronic hepatitis	Negative	5.3	23	H
11	71	F	Caucasian	Chronic hepatitis	Negative	6.2	87	F
12	51	M	Asian	Chronic hepatitis	Negative	5.7	435	C
13	52	M	Caucasian	Chronic infection	Negative	4.4	18	A
14	40	F	Caucasian	Chronic infection	Negative	4.2	29	D
15	33	M	Asian	Chronic infection	Negative	4.3	25	D
16	63	M	Hispanic	Cirrhosis	Negative	4.0	16	A
17	53	M	Caucasian	Cirrhosis/HCC	Negative	3.7	36	A
18	35	M	Sub-Saharan	Chronic hepatitis	Positive	5.7	36	E
19	37	M	Caucasian	Chronic hepatitis	Positive	8.4	32	C
20	45	M	Caucasian	Chronic hepatitis	Positive	5.6	35	A
21	29	F	Asian	Chronic hepatitis	Positive	6.9	355	B
22	28	M	Asian	Chronic hepatitis	Positive	> 8.0	341	C
23	28	M	Asian	Chronic infection	Positive	8.7	24	C
24	28	F	Asian	Chronic infection	Positive	8.8	22	C
25	55	F	Caucasian	Cirrhosis	Positive	5.4	73.9	A
26	82	F	Caucasian	Cirrhosis/HCC	Positive	4.8	24	C
27	64	M	Caucasian	Cirrhosis/HCC	Positive	6.3	45	D

¹Genotype determined by Sanger sequencing of the X region (same region as was analyzed by next-generation sequencing). ALT: Alanine aminotransferase; HBV: Hepatitis B virus; HBeAg: Hepatitis B e antigen; M: Male; F: Female; HBV: Hepatitis B virus.

Table 2 Results of genotyping of nucleotide haplotypes obtained in each patient, extracted by next-generation sequencing based on ultra-deep pyrosequencing analysis %

Patient	A	B	C	D	E	F	H
1	0	0	0	0	100	0	0
2	0	0	100	0	0	0	0
3	7.1	0	0	92.9	0	0	0
4	0	0	0	100	0	0	0
5	0	0	0	100	0	0	0
6	1.7	0	0	98.3	0	0	0
7	0	0	0.3	51.3	0	48.4	0
8	7.1	0	0	92.9	0	0	0
9	0	0	0.3	7	0	92.7	0
10	0.9	0	0	50.9	0	0	48.2
11	0	0	0.3	7	0	92.7	0
12	95.1	0	4.4	0.5	0	0	0
13	0	0	100	0	0	0	0
14	46.6	0	8.2	33.2	0	12	0
15	89.7	0	4.4	8	0	1.5	0
16	0	0	0	100	0	0	0
17	0	0	0	100	0	0	0
18	0	0	0	0	100	0	0
19	0	0	95.3	3,6	0.9	0	0
20	100	0	0	0	0	0	0
21	0	99.6	0.4	0	0	0	0
22	0	0	100	0	0	0	0
23	0	0	87.8	12.2	0	0	0
24	0	0	100	0	0	0	0
25	97.9	0	0	2.1	0	0	0
26	0	0	100	0	0	0	0
27	0	0	0	100	0	0	0

%A to %H indicates the percentage of nucleotide haplotypes from each patient, classified as HBV genotype A, B, C, D, E, F or H. HBV: Hepatitis B virus.