Copyright
©The Author(s) 2018.
World J Gastroenterol. Mar 28, 2018; 24(12): 1299-1311
Published online Mar 28, 2018. doi: 10.3748/wjg.v24.i12.1299
Published online Mar 28, 2018. doi: 10.3748/wjg.v24.i12.1299
Table 1 Sequence of primers used for adaptive mutation plasmid construction
| Primer | Sequence (5’-3’) |
| 1340-F | CTGGCGTACGTGATGCG |
| m2310-R | TGTCCCTGTCCTCCAAGCCGCAGCGAT |
| m2310-F | ATCGCTGCGGCTTGGAGGACAGGGACA |
| 3500-R | GCCCCGTCATACTCACCAC |
| m2681-R | TGATGTACCAAGCTGCCACGAAGAAG |
| m2681-F | CTTCTTCGTGGCAGCTTGGTACATCA |
| 5249-F | AATGAGGTCACCCTCACACA |
| m6132-R | ACGTGGCTTCCTCTGGAAGCAAAGGCA |
| m6132-F | TGCCTTTGCTTCCAGAGGAAGCCACGT |
| 7791-R | GATGTTGTACAGTACACCTTG |
| 5249-F | AATGAGGTCACCCTCACACA |
| m7160-R | AGCATGCGCTCCGATGGTATTGAG |
| m7160-F | CTCAATACCATCGGAGCGCATGCT |
| m7359-R | TTCTGATGTGGTGCTCTCGCTCAG |
| m7359-F | CTGAGCGAGAGCACCACATCAGAA |
| m7658-R | GCACAGGGTGGTATCGTCCTCCT |
| m7658-F | AGGAGGACGATACCACCCTGTGC |
| 7966-R | CTTGGATCTTGCAGAAT |
Table 2 Primer combinations used in adaptive mutation plasmid construction
| Fragment | Template | Primers | |
| Sense | Anti-sense | ||
| mE2-1 | JFH1 | 1340-F | m2310-R |
| mE2-2 | m2310-F | 3500-R | |
| mp7-1 | 1340-F | m2681-R | |
| mp7-2 | m2681-F | 3500-R | |
| mNS4B-1 | 5249-F | m6132-R | |
| mNS4B-2 | m6132-F | 7791-R | |
| mNS5A-1 | 5249-F | m7160-R | |
| mNS5A-2 | m7160-F | m7359-R | |
| mNS5A-3 | m7359-F | m7658-R | |
| mNS5A-4 | m7658-F | 7966-R | |
| mNS4B/NS5A-1 | JFH1-mNS5A | 5249-F | m6132-R |
| mNS4B/NS5A-2 | m6132-F | 7791-R | |
| mE2/p7 -1 | JFH1-mp7/NS5A | 1350-F | m2310-R |
| mE2/p7 -2 | m2310-F | 3500-R | |
Table 3 Primers and templates for overlap PCR
| Fragment | Template | Primer | |
| Upstream | Downstream | ||
| mE2 | mE2-1 + mE2-2 | 1340-F | 3500-R |
| mp7 | mp7-1 + mp7-2 | 1340-F | 3500-R |
| mNS4B | mNS4B-1 + mNS4B-2 | 5249 F | 7791-R |
| mNS5A-3/4 | mNS5A-3 + mNS5A-4 | m7359-F | 7966-R |
| mNS5A-2/3/4 | mNS5A-2 + mNS5A-3/4 | m7160-F | 7966-R |
| mNS5A | mNS5A-1 + mNS5A-2/3/4 | 5249-F | 7966-R |
| mNS4B/NS5A | mNS4B/NS5A-1 + mNS4B/NS5A-2 | 5249 F | 7791-R |
| mE2/p7 | mE2/p7 -1 + mE2/p7-2 | 1340-F | 3500-R |
- Citation: Wang Q, Li Y, Liu SA, Xie W, Cheng J. Cell culture-adaptive mutations in hepatitis C virus promote viral production by enhancing viral replication and release. World J Gastroenterol 2018; 24(12): 1299-1311
- URL: https://www.wjgnet.com/1007-9327/full/v24/i12/1299.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i12.1299