hsa-mir-183 is frequently methylated and related to poor survival in human hepatocellular carcinoma

Table 1 Clinopathological variables of hepatocellular carcinoma, hepatocellular adenoma, and focal nodular hyperplasia patients involved in this study

	HCC (n = 40)	HCA (n = 10)	FNH (n = 5)
Age
< 50	12	10	4
> 50	28	0	1
Sex
Male	33	1	2
Female	7	9	3
Etiology
HBV	8
HCV	4
No infection	28
Tumor differentiation
Good	15
Moderate	17
Poor	8
Tumor size
< 5 cm	20	4	4
> 5 cm	20	6	1
Stage
I	5
II	11
III	16
IV	8
Number of nodules
Unilocular	14	9	4
Multilocular	26	1	1
Cirrhosis
With cirrhosis	32
Without cirrhosis	8
Survival
< 3 yr	18
> 3 yr	17
No information	2
Diagnosed < 2 yr ago	3

HCC: Hepatocellular carcinoma; HCA: Hepatocellular adenoma; FNH: Focal nodular hyperplasia; HBV: Hepatitis B virus; HCV: Hepatitis C virus.

Table 2 List of primers used in this study

Pyrosequencing	Forward	Reverse	Ta (°C)	MgCl2 (mmol/L)	Sequencing
miR-23 cluster	TTTAAGTYGTGTGAAATTATGTGGTAG	ATAAACACCRAAAAACRAATCCA	60	2.5	TGGTAGTTTATGGTTGTGAG
miR-25 cluster	GTGYGGGTTAATYGGATAAGG	AAAAACCCRACRCCTACACTAC	60	2.5	GYGGGTTTAGAATGAGT
miR-183	GTTATTAATAGGAATGGGGTAG	AAACRACTCTCAACCTCCC	60	1.5	GAATGGGGTAGTTGAGGG