Effect of toll-like receptor 3 agonist poly I:C on intestinal mucosa and epithelial barrier function in mouse models of acute colitis

doi:10.3748/wjg.v23.i6.999

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 14, 2017; 23(6): 999-1009
Published online Feb 14, 2017. doi: 10.3748/wjg.v23.i6.999

Table 1 Primers used for real-time quantitative polymerase chain reaction analysis

Gene	Primers		Length, bp
zo-1	Forward	5’-TCATCCCAAATAAGAACAGAGC-3	198
zo-1	Reverse	5’-GAAGAACAACCCTTTCATAAGC-3’	198
Occludin	Forward	5’-CTTTGGCTACGGAGGTGGCTAT-3’	86
Occludin	Reverse	5’-CTTTGGCTGCTCTTGGGTCTG-3’	86
Claudin-1	Forward	5’-GCTGGGTTTCATCCTGGCTTCT-3’	110
Claudin-1	Reverse	5’-CCTGAGCGGTCACGATGTTGTC-3’	110
IL-17	Forward	5’-TATCCCTCTGTGATCTGGGAAG-3’	161
IL-17	Reverse	5’ATCTTCTCGACCCTGAAAGTGA-3’	161
IFN-γ	Reverse	5’-ATGAACGCTACACACTGCATCTT-3’	139
IFN-γ	Forward	5’-TTTCTTCCACATCTATGCCACTT3’	139
TNF-α	Reverse	5’-GGTTCTGTCCCTTTCACTCACT-3’	169
TNF-α	Forward	5’-GAGAAGAGGCTGAGACATAGGC-3’	169
GAPDH	Reverse	5’-GAGACCTTCAACACCCCAGC-3’	263
GAPDH	Forward	5’-ATGTCACGCACGATTTCCC-3’	263

IL-17: Interleukin 17; IFN-γ: Interferon-γ; TNF-α: tumor necrosis factor-α; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.

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Citation: Zhao HW, Yue YH, Han H, Chen XL, Lu YG, Zheng JM, Hou HT, Lang XM, He LL, Hu QL, Dun ZQ. Effect of toll-like receptor 3 agonist poly I:C on intestinal mucosa and epithelial barrier function in mouse models of acute colitis. World J Gastroenterol 2017; 23(6): 999-1009
URL: https://www.wjgnet.com/1007-9327/full/v23/i6/999.htm
DOI: https://dx.doi.org/10.3748/wjg.v23.i6.999