Copyright
©The Author(s) 2017.
World J Gastroenterol. Feb 14, 2017; 23(6): 964-975
Published online Feb 14, 2017. doi: 10.3748/wjg.v23.i6.964
Published online Feb 14, 2017. doi: 10.3748/wjg.v23.i6.964
Table 1 Primer sequences used in quantitative polymerase chain reaction analysis
| Gene | Primer sequences (5’-3’) | Annealing temperatures (°C) | |
| mLgr5 | F | ACATTCCCAAGGGAGCGTTC | 60 |
| R | ATGTGGTTGGCATCTAGGCG | ||
| mLyz1 | F | GCCAAGGTCTACAATCGTTGTGAGTTG | 60 |
| R | CAGTCAGCCAGCTTGACACCACG | ||
| mMuc2 | F | ATGCCCACCTCCTCAAAGAC | 60 |
| R | GTAGTTTCCGTTGGAACAGTGAA | ||
| mChgA | F | CCCACTGCAGCATCCAGTT | 60 |
| R | AGTCCGACTGACCATCATCTTTC | ||
| mALP | F | AACTCACCTCATGGGCCTCTT | 60 |
| R | GGGTTTCGGTTGGCATCATA | ||
| mGAPDH | F | TCATCAACGGGAAGCCCATCAC | |
| R | AGACTCCACGACATACTCAGCACCG | ||
- Citation: Han SH, Shim S, Kim MJ, Shin HY, Jang WS, Lee SJ, Jin YW, Lee SS, Lee SB, Park S. Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor. World J Gastroenterol 2017; 23(6): 964-975
- URL: https://www.wjgnet.com/1007-9327/full/v23/i6/964.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i6.964