Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights

Table 1 Assessment of colon lesions in rats subjected to obstruction of the left colic artery and vein by two ligations for 15 min (pale flat areas without mucosal folds as a percentage of the area of the blood-deprived colon segment; mean ± SD)

Medication (/kg, 1-ml bath/rat) at the 25-mm blood-flow-deprived colon segment	Pale areas without mucosal folds as a percentage of the area of the blood-deprived colon segment, mean ± SD
0.9% NaCl (control)	46 ± 8
BPC 157 (10 µg)	7 ± 2a
L-NAME (5 mg)	80 ± 10a
L-arginine (100 mg)	65 ± 6a
L-NAME (5 mg) + L-arginine (100 mg)	50 ± 8
L-NAME (5 mg) + BPC 157 (10 µg)	10 ± 4a
L-arginine (100 mg) + BPC 157 (10 µg)	6 ± 2a
L-NAME (5 mg) + L-arginine (100 mg) + BPC 157 (10 µg)	8 ± 2a

The following medications or treatments (/kg, 1-mL bath/rat) were applied to the 25-mm blood-flow-deprived colon segment at 1 min post-injury: BPC 157 (10 µg); the NOS blocker L-NAME (5 mg) and the NOS substrate L-arginine (100 mg), alone or combined; equal volume of saline bath (controls).

^aP < 0.05 at least vs control.

Table 2 Assessment of colon lesions in rats subjected to two ligations of the left colic artery and vein for 15 min followed by reperfusion for next 15 min (IC+RL rats) (pale flat areas without mucosal folds were measured and are shown as a percentage of the area of the blood-deprived and the reperfused colon segment; mean ± SD)

Medication (/kg, 1 ml bath/rat) applied to the 25-mm blood-flow-deprived and then reperfused colon segment	Pale areas without mucosal folds as a percentage of the area of the blood-flow-deprived and then reperfused colon segment (mean ± SD)
0.9% NaCl (control)	86 ± 8
BPC 157 (10 µg)	10 ± 2a

At 1 min reperfusion time, BPC 157 (10 µg/kg, 1 mL bath/rat) or an equal volume of saline bath (control) was applied to the 25-mm blood-flow-deprived colon segment.

^aP < 0.05 at least vs control.

Table 3 Assessment of lesions in rats with obstruction of the left colic artery and vein for 10 d via two ligations; the animals underwent additional colon obstruction for the first 3 d

Medication (/kg, 1 ml bath/rat) at the 25-mm blood-flow- deprived colon segment	Pale areas without mucosal folds as a percentage of the entire area of the blood-deprived colon segment means ± SD	Number of gross mucosal defects,mean ± SD	Colon diameter,mean ± SD	Adhesion severityscored 0-2,Min/Med/Max
0.9% NaCl (control)	125 ± 10	4 ± 1	36 ± 8	2/2/2002
BPC 157 (10 µg)	10 ± 2a	0 ± 0a	9 ± 1a	0/1/1a

They received BPC 157 or saline bath on day 3, immediately after the additional colon obstruction was removed. The animals were assessed one week thereafter, on day 10 after ligation. Pale flat areas without mucosal folds were measured and are expressed as the percentage of the entire area of the blood-deprived colon segment (mean ± SD). Number of gross mucosal defects; mean ± SD; colon diameter, mean ± SD; adhesion severity, scored 0-2 (Min/Med/Max, respectively).

^aP < 0.05 at least vs control.