Estrogen receptor expression in chronic hepatitis C and hepatocellular carcinoma pathogenesis

Table 1 Age ranges of normal and diseased subjects in whole tissue lysates

Group	No. of subjects	No. of females	Age range (yr)	No. of males	Age range (yr)
Normal	20	11	42-67	9	34-64
HCV	14	4	53-60	10	43-55
HCC	14	1	56	13	44-66

HCV: Hepatitis C virus; HCC: Hepatocellular carcinoma.

Table 2 Age ranges of normal and diseased subjects in mRNA analysis

Group	No. of subjects	No. of females	Age range (yrs)	No. of males	Age range (yrs)
Normal	9	8	42-67	1	64
HCV	10	3	43-60	7	43-53
HCC	10	0	N/A	10	44-59

HCV: Hepatitis C virus; HCC: Hepatocellular carcinoma.

Table 3 Age ranges of normal and diseased subjects in nuclear and cytoplasmic tissue lysates

Group	No. of subjects	No. of females	Age range (yr)	No. of males	Age range (yr)
Normal	10	5	43-67	5	50-64
HCV	12	3	51-60	9	46-53
HCC	16	0	NA	16	44-66

HCV: Hepatitis C virus; HCC: Hepatocellular carcinoma.

Table 4 Spearman correlations of ERα and ERβ expression in whole tissue lysates with nuclear cyclin D1, nuclear phosphorylated NF-κB and cytoplasmic phosphorylated IKK expression

	R-coefficient (P value)
	Normal	HCV	HCC
Correlations with WTL ERα
ERβ	0.4154 (0.0692)	0.81944 (0.0006)b	0.70339 (0.0107)a
Nuclear cyclin D1	0.62189 (0.0549)	0.88842 (0.0075)a	-0.29785 (0.3737)
Nuclear pNF-κB	0.02757 (0.9397)	0.84435 (0.0169)a	0.19351 (0.5686)
Cytoplasmic pIKK	-0.21577 (0.5494)	-0.09787 (0.8346)	-0.37679 (0.2534)
Correlations with WTL ERβ
Nuclear cyclin D1	-0.03251 (0.9290)	0.92648 (0.0027)a	-0.70135 (0.0162)a
Nuclear pNF-κB	-0.18056 (0.6176)	0.71732 (0.0696)	0.41196 (0.2080)
Cytoplasmic pIKK	0.24608 (0.4931)	-0.21801 (0.6386)	-0.03438 (0.9201)

^aP < 0.05,

^bP < 0.001. HCV: Hepatitis C virus; HCC: Hepatocellular carcinoma; ER: Estrogen receptor; WTL: Whole tissue lysates.

Table 5 Spearman correlations of ERα and ERβ expression in nuclear tissue lysates with nuclear cyclin D1 and nuclear phosphorylated NF-κB expression

	r-coefficient (P value)
	Normal	HCV	HCC
Correlations with nuclear ERα
Nuclear ERβ	0.63880 (0.0468)a	0.63539 (0.0264)a	0.73192 (0.0008)b
Nuclear cyclin D1	0.12049 (0.7402)	0.75728 (0.0043)a	0.55714 (0.0202)a
Nuclear pNF-κB	-0.62968(0.0511)c	0.207 (0.5186)	0.4793 (0.0516)c
Correlations with nuclear ERβ
Nuclear cyclin D1	0.44012 (0.2031)	0.64445 (0.0237)a	0.51223 (0.0355)a
Nuclear pNF-κB	-0.52091 (0.1226)	0.10243 (0.7514)	0.25861 (0.3162)

^aP < 0.05,

^bP < 0.001,

^cP < 0.055. HCV: Hepatitis C virus; HCC: Hepatocellular carcinoma; ER: Estrogen receptor.

Table 6 Spearman correlations of ERα and ERβ expression in cytoplasmic tissue lysates with cytoplasmic pIKK

	r-coefficient (P value)
	Normal	HCV	HCC
Correlations with cytoplasmic ERα
Cytoplasmic ERβ	0.61233 (0.0599)	0.74376 (< 0.0001)a	0.79765 (0.0158)b
Cytoplasmic pIKK	-0.66837 (0.0346)a	-0.00698 (0.9828)	-0.56014 (0.0194)a
Correlations with cytoplasmic ERβ
Cytoplasmic ERα	0.61233 (0.0599)	0.91363 (< 0.0001)b	0.79765 (0.0001)b
Cytoplasmic pIKK	-0.60909 (0.0616)	-0.29717 (0.3482)	-0.60848 (0.0095)a

^aP < 0.05,

^bP < 0.001. HCV: Hepatitis C virus; HCC: Hepatocellular carcinoma; ER: Estrogen receptor.