(-)-Epigallocatechin-3-gallate enhances poly I:C-induced interferon-λ1 production and inhibits hepatitis C virus replication in hepatocytes

doi:10.3748/wjg.v23.i32.5895

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 28, 2017; 23(32): 5895-5903
Published online Aug 28, 2017. doi: 10.3748/wjg.v23.i32.5895

Table 1 Primers used for real-time reverse transcription-polymerase chain reaction

Primer	Orientation	Sequences (5’-3’)
GAPDH	Forward	GGTGGTCTCCTCTGACTTCAACA
	Reverse	GTTGCTGTAGCCAAATTCGTTGT
IFN-λ1	Forward	CTTCCAAGCCCACCCCAACT
	Reverse	GGCCTCCAGGACCTTCAGC
TLR3	Forward	AGCCACCTGAAGTTGACTCAGG
	Reverse	CAGTCAAATTCGTGCAGAAGGC
RIG-I	Forward	CTTGGCATGTTACACAGCTGAC
	Reverse	GCTTGGGATGTGGTCTACTCA
IRF-9	Forward	GCATCAGGCAGGGCACGCTGCACCCG
	Reverse	GCCTGCATGTTTCCAGGGAATCCGG
ISG15	Forward	GGCTGGGAGCTGACGGTGAAG
	Reverse	GCTCCGCCCGCCAGGCTCTGT
ISG56	Forward	TTCGGAGAAAGGCATTAGA
	Reverse	TCCAGGGCTTCATTCATAT
MxA	Forward	GCCGGCTGTGGATATGCTA
	Reverse	TTTATCGAAACATCTGTGAAAGCAA
OAS-1	Forward	AGAAGGCAGCTCACGAAACC
	Reverse	CCACCACCCAAGTTTCCTGTA
HCV	Forward	RAYCACTCCCCTGTGAGGAAC
	Reverse	TGRTGCACGGTCTACGAGACCTC

HCV: Hepatitis C virus; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; IFN: Interferon; TLR3: Toll-like receptor 3; RIG-I: Retinoic acid-inducible gene I.

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Citation: Wang YZ, Li JL, Wang X, Zhang T, Ho WZ. (-)-Epigallocatechin-3-gallate enhances poly I:C-induced interferon-λ1 production and inhibits hepatitis C virus replication in hepatocytes. World J Gastroenterol 2017; 23(32): 5895-5903
URL: https://www.wjgnet.com/1007-9327/full/v23/i32/5895.htm
DOI: https://dx.doi.org/10.3748/wjg.v23.i32.5895