Schistosoma japonicum attenuates dextran sodium sulfate-induced colitis in mice via reduction of endoplasmic reticulum stress

Table 1 Disease activity index score parameters

Score	Body weight loss	Stool consistency	Rectal bleeding
0	0%-1%	Normal	None
1	1%-5%	Soft and shaped	Between
2	5%-10%	Loose	Slight
3	10%-15%	Between	Between
4	> 15%	Diarrhea	Gross bleeding

Table 2 Histological scoring system used in this study

Score	Damaged area	Mucodepletion of glands	Tissue damage	Inflammatory cell infiltration
0	N/A	None	No mucosal damage	Occasional inflammatory cells in the lamina propria
1	≤ 25%	Mild	Discrete epithelial lesions	Increased numbers of inflammatory cells in the lamina propria
2	≤ 50%	Moderate	Surface mucosal erosion or focal ulceration	Confluence inflammatory cells, extending into the submucosa
3	≤ 75%	Moderate	Extensive mucosal damage and extension into deeper structures of the bowel wall	Transmural extension of the infiltrate
4	≤ 100%	Severe

Table 3 Gene-specific primer pairs used in the study

Primer name		Primer sequence	Product length (bp)
M-GAPDH	Forward	5’-TGAAGGGTGGAGCCAAAAG-3’	227
	Reverse	5’-AGTCTTCTGGGTGGCAGTGAT-3’
M-IL-6	Forward	5’-CTTCTTGGGACTGATGCTGGT-3’	171
	Reverse	5’-CACAACTCTTTTCTCATTTCCACG-3’
M-IL-10	Forward	5’-TACAGCCGGGAAGACAATAACT-3’	142
	Reverse	5’-AGGAGTCGGTTAGCAGTATGTTG-3’
M-TNF-α	Forward	5`-TCCCCAAAGGGATGAGAAGTT-3’	298
	Reverse	5’-GAGGAGGTTGACTTTCTCCTGG-3’
M-TGF-β	Forward	5’-AGAGCCCTGGATACCAACTATTG-3’	286
	Reverse	5’-TGCGACCCACGTAGTAGACG-3’
M-IFN-γ	Forward	5’-CTCAAGTGGCATAGATGTGGAAG-’	250
	Reverse	5’-GACCTCAAACTTGGCAATACTCA-3’
M-IL-1b	Forward	5’-GGGCCTCAAAGGAAAGAATCT-3’	195
	Reverse	5’-GAGGTGCTGATGTACCAGTTGG-3’
M-IL-17a	Forward	5’-GTCTTTAACTCCCTTGGCGC-3’	136
	Reverse	5’-GGCACTGAGCTTCCCAGATC-3’
M-IRE1a	Forward	5’-ACACACCGACCACCGTATCTC-3’	157
	Reverse	5’-GGGTAAGTGATGATGAACGCC-3’
M-IRE1b	Forward	5’-TGGACGGTCCCACAACAGAT-3’	140
	Reverse	5’-GGGAGGTTCGTGGTATCCAA-3’
M-GRP78	Forward	5’-GATGAAATTGTTCTGGTTGGTGG-3’	203
	Reverse	5’-AGTGTAAGGGGACAAACATCAAG-3’
M-CHOP	Forward	5’-GGAGCTGGAAGCCTGGTATG-3’	285
	Reverse	5’-GGGCACTGACCACTCTGTTTC-3’