Clinical Trials Study
Copyright ©The Author(s) 2017.
World J Gastroenterol. Apr 14, 2017; 23(14): 2601-2612
Published online Apr 14, 2017. doi: 10.3748/wjg.v23.i14.2601
Table 1 Comparison of the clinicopathologic characteristics of gallbladder squamous/adenosquamous carcinomas and adenocarcinomas with the expression of ROR2 and WNT5a n (%)
Clinicopathologic characteristicSC/ASC,n = 46AC,n = 80χ2P value
Sex
Male19 (41.3)26 (32.5)0.9860.352
Female27 (58.7)54 (67.5)
Age, yr
≤ 453 (6.5)16 (20.0)4.1430.042
> 4543 (93.5)64 (80.0)
Degree of differentiation
High16 (34.8)27 (33.8)8.5150.014
Moderate24 (52.2)25 (31.3)
Poor6 (13.0)28 (35.0)
Maximal diameter of the mass, cm
≤ 320 (43.5)50 (62.5)4.2800.039
> 326 (56.5)30 (37.5)
Gallstones
No18 (39.1)42 (52.5)2.0930.148
Yes28 (60.9)38 (47.5)
TNM stage
I + II12 (26.1)21 (26.3)0.2870.866
III20 (33.5)38 (47.5)
IV14 (30.4)21 (26.3)
Lymph node metastasis
No17 (37.0)30 (37.5)0.0040.952
Yes29 (63.0)50 (62.5)
Invasion of the surrounding tissue
No16 (34.8)31 (38.8)0.1970.658
Yes30 (62.5)49 (61.3)
Surgical procedure
Radical14 (30.4)26 (32.5)0.2150.898
Palliative18 (39.1)28 (35.0)
Unresected14 (30.4)26 (32.5)
Average survival time10.07 (4-25)10.34 (3-27)0.0140.906
ROR2
-20 (43.5)29 (36.2)0.6420.386
+26 (56.5)51 (63.8)
WNT5a
-17 (37.0)31 (38.7)0.0400.858
+29 (63.0)49 (61.3)
Table 2 Relationship of ROR2 and WNT5a expression with the clinicopathologic characteristics of gallbladder squamous/adenosquamous carcinoma n (%)
Pathologic characteristicNumber of casesROR2
WNT5a
Positive casesχ2P valuePositive casesχ2P value
Pathological type
Squamous carcinoma2616 (61.5)0.6120.43417 (65.4)0.1410.708
Adenosquamous carcinoma2010 (50.0)12 (60.0)
Differentiation
High165 (31.3)9.1230.0108 (50.0)1.8270.401
Moderate2415 (62.5)17 (70.8)
Poor66 (100.0)4 (66.7)
Maximal diameter of the mass in cm
≤ 3207 (35.0)6.6690.0109 (45.0)4.9450.026
≥ 32619 (73.1)20 (76.9)
Gallstones
No1811 (61.1)0.2530.61512 (66.7)0.1670.683
Yes2815 (53.6)17 (60.7)
TNM stage
I + II124 (33.3)4 (33.3)
III2010 (50.0)7.8240.02314 (70.0)6.4110.041
IV1412 (85.7)11 (78.6)
Lymph node metastasis
No175 (29.4)8.0650.0057 (41.2)6.7200.010
Yes2921 (72.4)22 (75.9)
Invasion of the surrounding tissue
No165 (31.3)6.3760.0117 (43.8)3.9200.048
Yes3021 (70.0)22 (73.3)
Surgical procedure
Radical144 (28.6)7.3740.0225 (35.7)77.6770.019
Palliative1811 (61.1)12 (66.7)
Unresected1411 (78.6)12 (85.7)
Table 3 Relationship of ROR2 and WNT5a expression with the clinicopathologic characteristics of gallbladder adenocarcinomas n (%)
Pathologic characteristicNumber of casesROR2
WNT5a
Positive casesχ2P valuePositive casesχ2P value
Differentiation
High2712 (44.0)10.3520.00211 (40.7)8.4040.015
Moderate2515 (60.0)16 (64.0)
Poor2824 (85.7)22 (78.6)
Maximal diameter of the mass in cm
≤ 35026 (52.0)7.9660.00426 (52.0)4.8070.029
> 33025 (83.3)23 (76.7)
Gallstones
No4226 (61.9)0.130.71826 (61.9)0.0160.899
Yes3825 (65.8)23 (60.5)
TNM stage
I + II217 (33.3)7 (33.3)
III3825 (65.8)14.968024 (63.2)12.2480.002
IV2119 (90.5)18 (85.7)
Lymph node metastasis
No3012 (40.0)11.7160.00112 (40.0)9.1320.003
Yes5039 (78.0)37 (74.0)
Invasion of the surrounding tissue
No3113 (41.9)10.4220.00212 (38.7)10.8340.001
Yes4938 (77.6)37 (75.5)
Surgical procedure
Radical2610 (38.5)12.2960.00210 (38.5)11.680.003
Palliative2819 (67.9)17 (60.7)
Unresected2622 (84.6)22 (84.6)
Table 4 Relationship of squamous/adenosquamous carcinoma clinicopathological parameters and ROR2 and WNT5a expression with average survival
GroupingNumber of cases, nAverage survival in moχ2P value
Sex
Male1910.74 (6-24)0.7670.381
Female279.85 (4-24)
Age, yr
≤ 45315.67 (8-24)2.0230.155
> 45439.84 (4-25)
Pathological type
Squamous carcinoma2610.19 (4-24)0.2230.637
Adenosquamous carcinoma2010.25 (4-24)
Degree of differentiation
High1613.81 (5-24)19.1250.000
Moderate248.92 (4-18)
Poor65.83 (4-9)
Maximal diameter of the mass, cm
≤ 32014.35 (7-24)31.3370.000
> 3267.04 (4-11)
Gallstones
No188.22 (4-12)3.7300.053
Yes2811.50 (4-24)
TNM stage
I + II1217.00 (9-24)51.1390.000
III209.20 (7-15)
IV145.86 (4-8)
Lymph node metastasis
No1714.24 (4-24)16.2190.000
Yes297.86 (4-15)
Invasion of the surrounding tissue
No1615.75 (9-24)32.2710.000
Yes307.27 (4-12)
Surgical procedure
Radical1416.64 (10-24)50.1650.000
Palliative188.50 (6-12)
Unresected146.00 (4-8)
ROR2
-2013.65 (6-24)16.5020.000
+267.58 (4-15)
WNT5a
-1713.77 (6-24)10.8440.001
+298.14 (4-15)
Table 5 Multivariate Cox regression analysis for survival of patients with gallbladder squamous/adenosquamous carcinoma
GroupingFactorBSEWaldP valueRR95%CI
LowerUpper
Pathological typeSC/ASC0.0940.3460.0740.7861.0990.5582.164
Degree of differentiationHigh/Moderate/Poor0.8330.3695.0960.0242.3001.1164.741
Maximal diameter of the mass, cm≤ 3/> 32.3740.74310.2090.00110.7402.50446.075
GallstonesNo/Yes0.6410.4202.3290.1271.8980.8334.324
TNM stageI + II/III/IV1.3620.4768.1870.0043.9041.5369.924
Lymph node metastasisNo/Yes1.7920.56410.0950.0016.0111.98718.128
Invasion of the surrounding tissueNo/Yes2.6480.78211.4660.00114.1263.05065.413
Surgical procedureRadical/Palliative/Unresected1.1040.4815.2680.0223.0161.1757.743
ROR2-/+1.6230.6745.7990.0165.0681.35318.992
WNT5a-/+1.2310.4896.3370.0123.4251.3138.930
Table 6 Relationship of the adenocarcinomas’ clinicopathological parameters and ROR2 and WNT5a expression with average survival
GroupingNumber of cases, nAverage survival in moχ2P value
Sex
Male269.58 (3-24)2.5670.109
Female5411.30 (3-24)
Age, yr
≤ 451610.81 (4-24)0.0030.956
> 456410.72 (3-24)
Degree of differentiation
High2715.07 (5-24)32.5010.000
Moderate2510.60 (4-24)
Poor286.68 (3-14)
Maximal diameter of the mass, cm
≤ 35013.70 (6-24)68.2830.000
> 3305.80 (3-10)
Gallstones
No4210.19 (3-24)0.2460.620
Yes3811.34 (4-24)
TNM stage
I + II2118.96 (5-24)105.8250.000
III389.29 (6-15)
IV215.14 (3-7)
Lymph node metastasis
No3016.27 (4-24)42.3720.000
Yes507.42 (3-14)
Invasion of the surrounding tissue
No3116.68 (7-24)55.5350.000
Yes496.98 (3-11)
Surgical procedure
Radical2618.31 (10-24)113.1410.000
Palliative288.64 (6-11)
Unresected265.42 (3-9)
ROR2
-2915.93 (6-24)32.9940.000
+517.78 (3-19)
WNT5a
-3115.48 (4-24)31.6540.002
+497.74 (3-18)
Table 7 Multivariate Cox regression analysis for survival of patients with gallbladder adenocarcinomas
GroupingFactorBSEWaldP valueRR95%CI
LowerUpper
Degree of differentiationHigh/Moderate/Poor1.0970.4964.9820.0272.9951.1337.918
Maximal diameter of the mass, cm≤ 3/> 30.9850.4634.5260.0332.6781.0816.636
GallstonesNo/Yes0.4120.3811.1690.2801.5100.7163.186
TNM stageI + II/III/IV1.3960.4599.2500.0024.0391.6439.931
Lymph node metastasisNo/Yes1.3510.4658.4410.0043.8611.5529.606
Invasion of the surrounding tissueNo/Yes1.6440.5837.9520.0055.1761.65116.227
Surgical procedureRadical/Palliative/ Unresected1.7370.53110.7010.0015.6802.00616.083
ROR2-/+2.0230.6749.0090.0037.5612.01828.333
WNT5a-/+1.9310.6498.8530.0036.8961.93324.607