Clinical Trials Study
Copyright ©The Author(s) 2017.
World J Gastroenterol. Apr 14, 2017; 23(14): 2585-2591
Published online Apr 14, 2017. doi: 10.3748/wjg.v23.i14.2585
Table 1 Two sets of primers of exon 9 and 20 of PIK3CA gene for polymerase chain reaction
ExonPrimers
Exon 9Forward5’CAAAGCAATTTCTACACGAGATCC 3’
Reverse5’GTAAAAACATGCTGAGATCAGCCACAT 3’
Exon 20Forward5’TGGAATGCCAGAACTACAATCTTT 3’
Reverse5’GGTCTTTGCCTGCTGAGAGTT 3’
Table 2 Primers of PIK3CA gene for pyrosequencing
ExonPrimers
Exon 9 RS1Nucleotide dispensation order5’ CCATAGAAAATCTTTCTCCT 3’
5’ ATCGACTACACTGACTGACTGACTGACTGACTGACTG 3’
Exon 9 RS2Nucleotide dispensation order5’ TTCTCCTTGCTTCAGTGATTT 3’
5’ ATACACATGTCAGTCAGACTAGCTAGCTAGCTAG 3’
Exon 9 RS3Nucleotide dispensation order5’ TAGAAAATCTTTCTCCTGCT 3’
5’ ATAGCACTGACTGACTGACTACTGACTGACTGACTG 3’
Exon 20 RSNucleotide dispensation order5’TGGAATGCCAGAACTACAATCTTT 3’
5’GGTCTTTGCCTGCTGAGAGTT 3’
Table 3 PIK3CA mutations and clinicopathological characteristics in Northwest Chinese esophageal squamous cell carcinoma patients n (%)
Clinical, epidemiologic, or pathologic featureTotal, nPIK3CA
P value
MutantWild-type
All cases21048162
Sex0.4756
Male137 (65.3)34 (70.8)123 (75.9)
Female73 (34.7)14 (29.2)39 (24.1)
Tobacco use0.2684
Yes149 (71.0)31 (64.6)118 (72.9)
No61 (29.0)17 (35.4)44 (28.1)
Alcohol use0.3778
Yes175 (83.3)38 (79.2)137 (84.6)
No35 (16.7)10 (20.8)25 (15.4)
Preoperative treatment0.8467
Yes28 (13.3)6 (12.5)22 (13.6)
No182 (86.7)42 (87.5)140 (86.4)
Tumor location0.9651
Upper thoracic20 (9.5)5 (10.4)15 (9.2)
Middle thoracic109 (51.9)25 (52.1)84 (51.9)
Lower thoracic81 (38.6)18 (37.5)63 (38.9)
Stage0.1641
IA16 (7.6)3 (6.3)13 (8.0)
IB20 (9.5)5 (10.4)15 (9.3)
IIA28 (13.3)11 (22.9)17 (10.5)
IIB44 (21.0)10 (20.8)34 (21.0)
IIIA49 (23.3)12 (25.0)37 (22.8)
IIIB23 (11.0)1 (2.1)22 (13.6)
IIIC30 (14.3)6 (12.5)24 (14.8)
Lymph node metastasis0.2663
Yes121 (57.6)31 (64.6)90 (55.6)
No89 (42.4)17 (35.4)72 (44.4)
Local recurrence0.7368
Yes43 (20.5)9 (18.8)34 (21.0)
No167 (79.5)39 (81.2)128 (79.0)
Prognosis0.0885
Dead88 (41.9)15 (31.3)73 (45.1)
Survived122 (58.1)33 (68.7)89 (54.9)