May the assessment of baseline mucosal molecular pattern predict the development of gluten related disorders among microscopic enteritis?

Table 1 Primers and probes

Tissue transglutaminase 2

Primer Forward:ATAAGTTAGCGCCGCTCTCC

Primer Reverse: CGGTGGCTCCTTCCACTG

Probe: GCCAGCCGCCAGTG

Interferon gamma

Primer Forward: CGCTTTACTTTATAGAAAACCTGGA

Primer Reverse: TCAATGAAGAGAACTTGGTCATTC

Probe: GCTTGAATCTAAA

Toll-like receptor 2

Primer Forward: CAAGATTCAAAGTATTTA

Primer Reverse: CCAGGTG CATTTAAAGA

Probe: TGCCCCTACTCAATCT

MyD88

Primer Forward: CAAGGCCTTGTCCCTGC

Primer Reverse: TCTGCCCTGCCTCCT

Probe: AGGCCCTGGGTGTGTGT

Table 2 Main demographic, clinical and pathologic features of enrolled patients

	Celiac disease(n = 31)	Gluten sensitivity(n = 17)	Irritable bowel syndrome(n = 29)	Other ME(n = 12)	Negative controls(n = 15)	P value
Age	34 ± 12	34.3 ± 6.1	36.2 ± 13.4	34.7 ± 14.2	32.6 ± 9.5	0.891
Sex (F/M)	19/12	15/2	22/7	11/1	9/6	0.062
IELs count	51.6 ± 10.6	18.6 ± 4.9	15.5 ± 5.1	19.0 ± 7.6	5.3 ± 1.5	< 0.0011
Weight loss	19 (61.3)	7 (41.2)	4 (13.8)	6 (50)	0	0.0022
Abdominal pain	25 (80.6)	16 (94.1)	28 (96.5)	8 (66.6)	0	0.042
Diarrhea	21 (67.7)	6 (35.3)	29 (100)	6 (50)	0	< 0.0012
Weakness	14 (45.1)	8 (47.0)	8 (27.6)	3 (25)	0	0.092
Headache	2 (6.5)	7 (41.2)	4 (13.8)	0 (0)	0	0.732
DQ 2-8	29 (93.5)	10 (58.8)	11 (37.9)	0 (0)	NA	< 0.0012
Iron deficiency anemia	8 (25.8)	2 (11.8)	2 (6.9)	8 (66.6)	0	0.632

¹ANOVA test;

²χ² test for trend. NA: Not available.