Copyright
©The Author(s) 2016.
World J Gastroenterol. Jul 14, 2016; 22(26): 6016-6026
Published online Jul 14, 2016. doi: 10.3748/wjg.v22.i26.6016
Published online Jul 14, 2016. doi: 10.3748/wjg.v22.i26.6016
Table 1 Characteristics of the primer pairs used for reverse transcription-quantitative real-time PCR analysis
| Primer name | Primer sequence (5’→3’) | Annealing temperature (°C) | Product lenght (bp) | Accession ID |
| GAPDH-F | GACCCCTTCATTGACCTCAAC | 60 | 136 | DQ403053 |
| GAPDH- R | CGCTCCTGGAAGATGGTGATGGG | |||
| PPARα-F | CCCCACTTGAAGCAGATGACC | 60 | 139 | NM_013196 |
| PPARα-R | CCCTAAGTACTGGTAGTCCGC | |||
| PPARδ-F | AATGCCTACCTGAAAAACTTCAAC | 60 | 96 | AJ306400 .1 |
| PPARδ-R | TGCCTGCCACAGCGTCTCAAT | |||
| PPARγ-F | CGGAGTCCTCCCAGCTGTTCGCC | 60 | 116 | Y12882 |
| PPARγ-R | GGCTCATATCTGTCTCCGTCTTC | |||
| CPT1-F | CCGCTCATGGTCAACAGCA | 60 | 105 | NM_031559 |
| CPT1-R | CAGCAGTATGGCGTGGATGG | |||
| Atg7-F | CCTCAGCGGATGTATGGACC | 60 | 160 | NM_001012097.1 |
| Atg7-R | AGCCACATTACACCCCAAGG |
- Citation: Vecchione G, Grasselli E, Voci A, Baldini F, Grattagliano I, Wang DQ, Portincasa P, Vergani L. Silybin counteracts lipid excess and oxidative stress in cultured steatotic hepatic cells. World J Gastroenterol 2016; 22(26): 6016-6026
- URL: https://www.wjgnet.com/1007-9327/full/v22/i26/6016.htm
- DOI: https://dx.doi.org/10.3748/wjg.v22.i26.6016