Clinical significance of HOTAIR expression in colon cancer

Table 1 Primers used for quantitative polymerase chain reaction

Primer	Sequence
HOTAIR
Forward	5'-CAGTGGGGAACTCTGACTCG-3'
Reverse	5'-GTGCCTGGTGCTCTCTTACC-3'
GAPDH
Forward	5'-GTCAACGGATTTGGTCTGTATT-3'
Reverse	5'-AGTCTTCTGGGTGGCAGTGAT-3'

Table 2 Relationship between HOTAIR expression and clinicopathological parameters in colon cancer

Clinicopathological parameter	No. of cases	HOTAIR expression	P value
Age (yr)
< 6	49	3.69 ± 1.94	0.188
≥ 60	31	3.45 ± 1.55
Gender
Male	43	3.91 ± 1.85	0.761
Female	37	3.23 ± 1.68
Tumor size (cm)
< 7	38	3.59 ± 1.59	0.599
≥ 7	42	3.60 ± 1.81
Lymph node metastasis
Yes	48	4.27 ± 1.54	0.024
No	32	3.11 ± 1.92
Tumor differentiation
High and moderate	41	2.93 ± 1.62	0.019
Low and undifferentiated	39	4.35 ± 1.82
TNM stage
I + II	46	3.17 ± 1.77	0.034
III + IV	34	3.87 ± 1.66

Table 3 Cox multiple regression analysis of risk factors for prognosis of colon cancer patients

Variable	Regression coefficient	SE	χ2	P value	OR	95%CI for OR
						Lower	Upper
TNM stage	0.732	0.345	4.489	0.034	2.0790	1.056	4.090
Lymph node metastasis	-2.512	1.088	5.325	0.021	0.0081	0.010	0.685
HOTAIR expression	-2.048	0.785	6.806	0.090	0.1290	0.028	0.601