Copyright
©The Author(s) 2016.
World J Gastroenterol. May 14, 2016; 22(18): 4567-4575
Published online May 14, 2016. doi: 10.3748/wjg.v22.i18.4567
Published online May 14, 2016. doi: 10.3748/wjg.v22.i18.4567
Table 1 Sequences of specific primers used for determining gene methylation status
Primer name | Forward primer sequence (5'-3') | Reverse primer sequence (5'-3') | Product size (bp) | AT (°C) | No. of cycles | |
DAPK | M | GGATAGTCGGATCGAGTTAACGTC | CCCTCCCAAACGCCGA | 98 | 60 | 35 |
U | GGAGGATAGTTGGATTGAGTTAATGTT | CAAATCCCTCCCAAACACCAA | 98 | 60 | 35 | |
p14 | M | GTGTTAAAGGGCGGCGTAGC | AAAACCCTCACTCGCGACGA | 122 | 60 | 40 |
U | TTTTTGGTGTTAAAGGGTGGTGTAGT | CACAAAAACCCTCACTCACAACAA | 132 | 60 | 40 | |
CDH1 | M | TTAGGTTAGAGGGTTATCGCGT | TAACTAAAAATTCACCTACCGAC | 115 | 53 | 35 |
U | TAATTTTAGGTTAGAGGGTTATTGT | CACAACCAATCAACAACACA | 97 | 57 | 35 | |
THBS1 | M | TGCGAGCGTTTTTTTAAATGC | TAAACTCGCAAACCAACTCG | 74 | 62 | 40 |
U | GTTTGGTTGTTGTTTATTGGTTG | CCTAAACTCACAAACCAACTCA | 115 | 62 | 40 |
Table 2 Association of Helicobacter pylori cagA+ infection as detected by PCR, with DNA methylation of the promoter regions of target genes in the esophageal biopsies
Table 3 Bivariate analysis of the association between molecular markers and the presence of Helicobacter pylori cagA+ infection in esophageal biopsies
Table 4 Association of Helicobacter pylori cagA+ infection as detected by PCR with DNA methylation of the promoter regions of target genes in 33 esophageal and gastric biopsies
Helicobacter pylori infection by PCR | P value | |||
Negative | Positive | |||
Esophageal biopsies | ||||
DAPK | Unmethylated | 14 | 0 | 0.0041 |
Methylated | 10 | 9 | ||
THBS1 | Unmethylated | 15 | 2 | 0.0571 |
Methylated | 9 | 7 | ||
CDH1 | Unmethylated | 11 | 1 | 0.107 |
Methylated | 13 | 8 | ||
p14 | Unmethylated | 18 | 5 | 0.400 |
Methylated | 6 | 4 | ||
Gastric biopsies | ||||
DAPK | Unmethylated | 17 | 6 | 0.444 |
Methylated | 6 | 4 | ||
THBS1 | Unmethylated | 15 | 3 | 0.062 |
Methylated | 7 | 7 | ||
CDH1 | Unmethylated | 6 | 1 | 0.397 |
Methylated | 17 | 9 | ||
p14 | Unmethylated | 14 | 8 | 0.440 |
Methylated | 8 | 2 |
Table 5 Clinical and histopathological data of patients depending on the presence of Helicobacter pylori cagA+ infection by PCR, in esophageal biopsies
Helicobacter pylori infection by PCR | P value | |||
Negative | Positive | |||
Age (yr) | ≤ 40 | 16 | 9 | 0.390 |
41-65 | 14 | 4 | ||
> 65 | 20 | 5 | ||
Gender | Feminine | 26 | 7 | 0.340 |
Masculine | 24 | 11 | ||
Helicobacter pylori (HE) | Negative | 38 | 8 | 0.0141 |
Positive | 12 | 10 | ||
Sydney Classification: Helicobacter pylori | Normal | 38 | 8 | 0.0141 |
Mild | 11 | 6 | ||
Moderate | 1 | 2 | ||
Marked | 0 | 2 | ||
Sydney Classification: Neutrophils | Normal | 23 | 5 | 0.0281 |
Mild | 25 | 8 | ||
Moderate | 1 | 4 | ||
Marked | 1 | 1 | ||
Sydney Classification: Mononuclear cells | Mild | 17 | 1 | 0.0211 |
Moderate | 30 | 13 | ||
Marked | 3 | 4 |
- Citation: Herrera-Goepfert R, Oñate-Ocaña LF, Mosqueda-Vargas JL, Herrera LA, Castro C, Mendoza J, González-Barrios R. Methylation of DAPK and THBS1 genes in esophageal gastric-type columnar metaplasia. World J Gastroenterol 2016; 22(18): 4567-4575
- URL: https://www.wjgnet.com/1007-9327/full/v22/i18/4567.htm
- DOI: https://dx.doi.org/10.3748/wjg.v22.i18.4567