Phosphatase and tensin homolog is a differential diagnostic marker between nonalcoholic and alcoholic fatty liver disease

Table 1 Demographic data, histological diagnosis of liver biopsies and phosphatase and tensin homolog expression in patients with non-alcoholic fatty liver disease

Case	Age/sex	BMI (kg/m2)	Lobular inflammation	Hepatocyte ballooning	Steatosis score	Steatosis	Fibrosis	NAS score	Scores for PTEN expression
									NS	S	NS-S
Controls
1	86/M	ND	0	0	0	0%	0	0	4	/	/
2	32/F	ND	0	0	0	0%	0	0	4	/	/
3	36/M	ND	0	0	0	0%	0	0	3	/	/
Pure steatosis
4	26/M	39	0	0	1	10%	0	1	4	2	2
5	46/M	43	0	0	1	30%	0	1	3	2	1
6	38/F	55	0	0	1	20%	0	1	4	2	2
7	36/F	34	0	0	1	10%	0	1	4	4	0
8	37/F	35	0	0	2	60%	0	2	3	1	2
9	45/M	54	0	0	2	50%	0	2	4	1	3
10	29/M	41	0	0	2	40%	0	2	4	1	3
11	60/F	39	0	0	3	80%	0	3	4	2	2
12	43/M	40	0	0	3	70%	0	3	4	1	3
13	45/M	42	0	0	3	70%	0	3	4	1	3
Steatohepatitis without fibrosis
14	49/F	36	0	1	1	30%	0	2	2	2	0
15	48/F	58	0	1	2	50%	0	3	4	1	3
16	57/M	34	1	1	2	60%	0	4	3	3	0
17	56/F	47	0	1	3	70%	0	4	4	2	2
18	40/M	40	0	1	3	70%	0	4	3	2	1
19	38/F	45	1	2	3	80%	0	6	3	1	2
20	59/F	41	1	1	3	70%	0	5	4	1	3
21	50/M	50	1	1	3	70%	0	5	4	1	3
22	35/F	51	1	1	3	80%	0	5	3	2	1
23	54/F	44	1	1	2	50%	0	4	3	2	1
24	42/F	39	1	1	3	80%	0	5	4	1	3
25	51/F	43	1	2	2	50%	0	5	4	2	2
26	56/M	39	1	1	3	80%	0	5	3	2	1
27	39/F	40	1	1	3	70%	0	5	3	2	1
Steatohepatitis with fibrosis
28	62/M	39	0	0	3	80%	1a	3	4	1	3
29	47/F	47	0	0	2	50%	1a	2	4	1	3
30	21/M	40	0	0	3	80%	1a	3	3	2	1
31	50/F	42	0	0	3	70%	1a	3	4	2	1
32	54/M	32	0	0	1	10%	1a	1	3	2	1
33	57/M	51	0	0	2	50%	1b	2	4	1	3
34	60/M	40	0	0	1	20%	1b	1	3	3	0
35	48/F	63	0	0	3	80%	1c	3	4	1	3
36	55/M	35	0	0	2	50%	2	2	4	1	3
37	40/F	45	0	0	3	70%	2	3	2	1	1
38	36/M	41	1	2	3	90%	3	6	4	1	3
39	59/M	36	1	2	2	60%	3	5	4	1	3
Cirrhosis
40	61/M	ND	0	1	1	20%	4	2	3	3	0
41	51/F	38	1	0	1	20%	4	2	3	3	0
42	54/M	58	1	1	2	50%	4	4	4	1	3
43	64/F	35	3	2	1	20%	4	6	3	2	1
44	60/M	36	1	2	2	50%	4	5	2	1	1
45	68/F	48	2	1	2	50%	4	5	3	1	2
46	65/F	47	1	1	3	70%	4	5	4	1	3
47	54/F	42	2	1	3	70%	4	6	4	1	3

The intensity of the PTEN nuclear staining was scored in steatotic (S) and non-steatotic (NS) areas of biopsies. All slides from healthy (Controls) and obese patients were scored on a staining intensity scale from 0 to 4 with score = 4 representing the highest intensity. Difference between NS and S area (NS-S) was calculated. ND: Not determined; PTEN: Phosphatase and tensin homolog.

Table 2 Demographic data, histological diagnosis of liver biopsies and phosphatase and tensin homolog expression in patients with alcoholic liver disease

Case	Age/sex	BMI (kg/m2)	Inflammation	Fibrosis	Cirrhosis	Steatosis	Score for PTEN expression
							NS	S	NS-S
Non-obese patients
1	56/F	26	-	p	-	100%	3	2	1
2	49/F	25	-	psn	-	40%	4	3	1
3	59/M	29	+	-	+	60%	3	3	0
4	57/F	27	+	-	+	80%	3	3	0
5	66/M	25	+	-	+	90%	4	3	1
6	55/F	28	+	-	+	90%	3	3	0
7	61/M	25	+	psn	+	70%	4	4	0
8	43/M	23	+	psn	+	80%	4	3	1
9	36/M	25	+	-	+	60%	3	2	1
10	41/M	24	+	psn	+	40%	4	3	1
11	64/M	21	+	-	+	10%	4	4	0
12	46/F	27	-	-	+	90%	3	3	0
13	28/F	22	+	-	+	80%	4	4	0
14	51/M	25	-	p	+	90%	4	3	1
15	43/M	20	+	psn	+	70%	3	3	0
16	54/M	20	-	psn	+	80%	4	4	0
17	36/M	23	-	psn	+	70%	3	2	1
Obese patients
18	60/M	30	-	psn	-	70%	3	3	0
19	55/F	41	-	p	-	70%	3	3	0
20	57/M	33	+	-	+	80%	3	3	0
21	55/M	30	+	-	+	70%	3	2	1
22	51/M	32	-	-	+	20%	4	4	0
23	65/M	31	-	psn	+	5%	4	4	0
24	72/M	34	+	psn	+	90%	4	4	0
25	39/M	31	+	-	+	20%	4	4	0

The intensity of the PTEN staining was scored in steatotic (S) and non-steatotic (NS) areas of liver sections. All liver sections from ALD patients were scored on a staining intensity scale from 0 to 4 with score = 4 representing the highest intensity. Difference between NS and S area (NS-S) was calculated. ND: Not determined; Ctrl: Controls; psn: Perisinusoidal; p: Portal; PTEN: Phosphatase and tensin homolog; ALD: Alcoholic liver disease.