Semaphorin 4D and hypoxia-inducible factor-1α overexpression is related to prognosis in colorectal carcinoma

Table 1 Correlation between semaphorin 4D and hypoxia-inducible factor-1α expression in colorectal carcinoma n (%)

	n	Sema4D-negative	Sema4D-positive
HIF-1α-negative	36	26 (72.2)	10 (27.8)
HIF-1α-positive	50	8 (16.0)	42 (84.0)

HIF-1α: Hypoxia-inducible factor-1 alpha; Sema4D: Semaphorin 4D.

Table 2 Expression of semaphorin 4D and hypoxia-inducible factor-1α and clinicopathological characteristics of colorectal carcinoma patients

		HIF-1α-positive			Sema4D-positive
	n	n (%)	χ2	P value	n (%)	χ2	P value
Age (yr)			0.013	0.911		0.130	0.719
≤ 50	40	23 (58)			25 (63)
> 50	46	27 (59)			27 (59)
Differentiation			8.5791	0.0031		6.6641	0.0101
High	28	10 (36)			11 (39)
Medium	42	30 (71)			28 (67)
Low	16	10 (63)			13 (81)
Tumor size			0.225	0.636		0.624	0.429
< 5 cm	50	28 (56)			32 (64)
≥ 5 cm	36	22 (61)			20(56)
TNM Stage			8.728	0.003		5.657	0.017
I-II	30	11 (37)			13 (43)
III-IV	56	39 (70)			39 (70)
Lymphatic metastasis			8.579	0.003		5.385	0.020
Yes	58	40 (69)			38 (66)
No	28	10 (36)			14 (50)

¹Represent a significant correlation between the differentiation status of the tumor. HIF-1α: Hypoxia-inducible factor-1α; Sema4D: Semaphorin 4D.

Table 3 Survival analysis of prognosis factors in colorectal carcinoma

		5-yr survival rate
	n	n (%)	χ2	P value
Age (yr)			1.664	0.197
≤ 50	40	12 (30)
> 50	46	20 (43)
Differentiation			4.7571	0.0291
High	28	15 (54)
Medium	42	13 (31)
Low	16	4 (25)
Tumor size			0.058	0.810
< 5 cm	50	18 (36)
≥ 5 cm	36	14 (39)
TNM stage			5.127	0.024
I-II	30	16
III-IV	56	16
Lymphatic metastasis			7.061	0.008
Yes	58	16 (28)
No	28	16 (57)
HIF-1α			8.920	0.003
Negative	36	20 (56)
Positive	50	12 (24)
Sema4D			11.244	0.001
Negative	34	20 (59)
Positive	52	12 (23)

¹Represent a significant correlation between the differentiation status of the tumor; HIF-1α: Hypoxia-inducible factor-1 alpha; Sema4D: Semaphorin 4D.

Table 4 Multivariate analysis of prognostic factors

	Overall survival
	HR	95%CI	P value
Differentiation	0.653	0.373-1.144	0.136
Tumor size	1.315	0.859-2.012	0.208
TNM stage	0.920	0.529-1.601	0.768
Lymphatic metastasis	2.499	1.345-4.641	0.004
HIF-1α	1.802	0.9.0-3.489	0.081
Sema4D	0.780	0.402-1.513	0.462
	2.147	1.157-3.983	0.015

HIF-1α: Hypoxia-inducible factor-1 alpha; Sema4D: Semaphorin 4D.