In vitro identification of nonalcoholic fatty liver disease-related protein hnRNPM

Table 1 Identification of intracellular glyceraldehyde-derived advanced glycation end-products formed by high fructose exposure

Spot No.	Protein name	Score	Mass values matched	Coverage	Calculated isoelectric point	Nominal mass (kDa)
1	Heterogeneous nuclear ribonucleoprotein M	90	13	20%	8.8	78
2	Heterogeneous nuclear ribonucleoprotein M	99	18	27%	8.8	78
3	Heterogeneous nuclear ribonucleoprotein M	84	16	28%	8.8	78
4	Heterogeneous nuclear ribonucleoprotein M	76	14	22%	8.8	78

Selected spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis. The number and the position of the four selected spots refer to the numbered spots in Figure 2B.

Table 2 Top 50 RNA targets of heterogeneous nuclear ribonucleoprotein M in Hep3B cells

Rank	Gene title	Gene symbol
1	Apolipoprotein E	APOE
2	Albumin	ALB
3	RNA, 28S ribosomal 1	RN28S1
4	PQ loop repeat containing 2	PQLC2
5	Zinc finger protein 865	ZNF865
6	Unknown	A_33_P3396434
7	lincRNA	XLOC_l2_004940
8	CD63 molecule	CD63
9	Eukaryotic translation elongation factor 1 alpha 1	EEF1A1
10	G protein-coupled receptor 155	GPR155
11	Family with sequence similarity 74, member A4	FAM74A4
12	LOC284600	ENST00000448179
13	LOC100506453	ENST00000381105
14	ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G	ATP5L
15	Unknown	A_33_P3370515
16	Proteasome (prosome, macropain) 26S subunit, non-ATPase, 2	PSMD2
17	Ribosomal protein L23	RPL23
18	Proteasome (prosome, macropain) subunit, beta type, 1	PSMB1
19	Arsenic (+3 oxidation state) methyltransferase	AS3MT
20	Heat shock 60 kDa protein 1 (chaperonin)	HSPD1
21	H2A histone family, member V	H2AFV
22	lincRNA	XLOC_l2_015885
23	Fucosyltransferase 6 (alpha (1,3) fucosyltransferase)	FUT6
24	Cytochrome P450, family 2, subfamily W, polypeptide 1	CYP2W1
25	Unknown	A_33_P3275826
26	Microtubule-associated protein 4	MAP4
27	lincRNA	XLOC_l2_002910
28	X-ray repair complementing defective repair in Chinese hamster cells 6	XRCC6
29	Solute carrier family 16, member 12 (monocarboxylic acid transporter 12)	SLC16A12
30	Actin, beta	ACTB
31	Unknown	A_33_P3315763
32	Mitochondrial carrier 1	MTCH1
33	Stromal cell-derived factor 2-like 1	SDF2L1
34	Chromosome 19 open reading frame 10	C19orf10
35	Kinesin family member 1C	KIF1C
36	Fibrinogen-like 1	FGL1
37	RAN, member RAS oncogene family	RAN
38	Unknown	ENST00000400768
39	Homer homolog 3 (Drosophila)	HOMER3
40	HscB iron-sulfur cluster co-chaperone homolog (E. coli)	HSCB
41	Canopy 2 homolog (zebrafish)	CNPY2
42	Thymidine phosphorylase	TYMP
43	F11 receptor	F11R
44	Mastermind-like 1 (Drosophila)	MAML1
45	Electron-transfer-flavoprotein, beta polypeptide	ETFB
46	Heterogeneous nuclear ribonucleoprotein A1	HNRNPA1
47	Alpha-1-microglobulin/bikunin precursor	AMBP
48	Calreticulin	CALR
49	Solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3	SLC25A3
50	Alpha-2-HS-glycoprotein	AHSG

RNA-hnRNPM complexes were co-immunoprecipitated with anti-heterogeneous nuclear ribonucleoprotein M antibody bound to protein G-magnetic beads, and RNAs were isolated and identified by microarray analysis.