Hepatic differentiation of rat induced pluripotent stem cells in vitro

doi:10.3748/wjg.v21.i39.11118

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Oct 21, 2015 (publication date) through Jan 11, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Gastroenterol. Oct 21, 2015; 21(39): 11118-11126
Published online Oct 21, 2015. doi: 10.3748/wjg.v21.i39.11118

Table 1 Primers, GenBank accession numbers, and amplicon size of genes for reverse transcription-polymerase chain reaction

Gene	Primer	Accession Number	Amplicon size (bp)
FOXA2	TGTCAGGAGCACAAGCGAGG (F)	NM-012743.1	194 bp
	GGGTGGTTGAAGGCGTAATGGTG (R)
SOX17	GGCACGGAACCCAACCAGC (F)	NM-001107902.1	119 bp
	CAGTCGTGTCCCTGGTAGGGAAGAC (R)
AFP	TCTGAAACGCCATCGAAATGCC (F)	NM-012493.2	285 bp
	AATGTAAATGTCGGCCAGTCCCT (R)
ALB	GGCACAGTGCTTGCAGAATTTCAG (F)	NM-134326.2	272 bp
	CACAGACGGTTCAGGATGGCAG (R)
CK8	TGAAGGATGCCAATGCCAAGCTG (F)	NM-199390.1	237 bp
	AACTCAGTCCTCCTGCGTAGCC (R)
CK18	ACGATTTCAGTCTCAACGACGCC (F)	NM-053976.1	147 bp
	TCCCTTCTTCTGAGCCTTAGTGCC (R)
CK19	GCTCAGCATGAAAGCTGCCCT (F)	NM-199498.1	299 bp
	CGCACTGGTAGCAAGGTAGGAG (R)
HNF4α	GATGTGCTGCTCCTAGGCAATGAC (F)	NM-001270931.1	261 bp
	CTGCCGGTCGTTGATGTAATCCTC (R)
GADPH	CCTTCATTGACCTCAACTAC (F)	NM-017008.4	594 bp
	GGAAGGCCATGCCAGTGAGC (R)

Citation: Sun C, Hu JJ, Pan Q, Cao Y, Fan JG, Li GM. Hepatic differentiation of rat induced pluripotent stem cells in vitro. World J Gastroenterol 2015; 21(39): 11118-11126
URL: https://www.wjgnet.com/1007-9327/full/v21/i39/11118.htm
DOI: https://dx.doi.org/10.3748/wjg.v21.i39.11118