Hes1, an important gene for activation of hepatic stellate cells, is regulated by Notch1 and TGF-β/BMP signaling

Table 1 Primers for reverse transcriptase-polymerase chain reaction

Gene	Forward primer	Reverse primer	Bp	Accession No.
Notch1	AGAGCTTTTCCTGTGTCTGTCC	CGGTACAGTCAGGTGTGTTGTT	414	NM_001105721
Notch2	GACTGCCAATACTCGACCTC	TTCAGAAGTGAAGTCTCCAG	438	NM_024358.1
Notch3	CCTCTTTCACCTGTACCTGTCC	ACACAGTAGTGGGAGTGGTCCT	496	NM_020087
Notch4	CAACTCTGCGAGAACGGTGG	TGGAAGGAGCCCAAGGTGTT	443	NM_001002827.1
hes1	GCCAGCTGATATAATGGA	TAGGTCATGGCGTTGATC	462	NM_024360.3
hey1	CTACAGCTCCTCTGATAGTG	GAGGCATCGAGTCCTTCAAT	420	NM_001191845
TGF-β1	GGTGAAACGGAAGCGCATCG	CTTGAATCTCTGCAGGCGCA	364	NM_021578.2
E-cadherin	CTCGTGGCTTTGTCAGCA	GACCCAGTCTCGTTTCTG	432	NM_031334.1
α-SMA	GTGTGAAGAGGAAGACAG	TTGGCCTTAGGGTTCAGC	348	NM_031004.2
β-catenin	CTGACAGAGTTGCTCCACTC	CAGCCCATCAACTGGATAGT	342	NM_053357.2
Bmp7	CAGAGCATCAACCCCAAGTT	GATGAAGTGAACCAGTGTCT	396	NM_001191856.1
COL1α2	ACCTCAGGGTGTTCAAGGTG	CGGATTCCAATAGGACCAGA	222	NM_053356
GAPDH	ACCACAGTCCATGCCATCAC	TCCACCACCCTGTTGCTGTA	480	NM_017008.4