Hepatocyte nuclear factor 4α induces a tendency of differentiation and activation of rat hepatic stellate cells

Table 1 Primer sequences used to identify the transformation of the immune phenotype during hepatocyte nuclear factor 4α-induced differentiation of rat hepatic stellate cells-T6 cells

Classification	Molecules	Sequence (Primer sequences)
Stem cell-related	CD133	F: 5′-TTAATGCAGCACCAGGTACATC-3′
		R: 5′-TCGTTGAGCAGGTAGGGAGTAT-3′
	CD105	F: 5′-ATCCCTCTGACCAGTGATGTCT-3′
		R: 5′-CTTTTTCCGAAGTGGTGGTAAG-3′
	Nestin	F: 5′-GAGTGTCGCTTAGAGGTGCAA-3′ R: 5′-TGTCACAGGAGTCTCAAGGGTA-3′
Hepatocyte	ALB	F: 5′-TGCAGGCTTGCTGTGATAAG-3′
differentiation-related		R: 5′-AGTAATCGGGGTGCCTTCTT-3′
	AFP	F: 5′-TACGTCCCTCCACCATTCTC-3′
		R: 5′-ATCCTGGTCTTTGCAGCACT-3′
	G-6-P	F: 5′-AAGAGGGCATAGCCCAGACT-3′
		R: 5′-TTGGAAGCTTCGTTGGTCTT-3′
	PEPCK	F: 5′-CAGGTTCCCAAAGGTCTGAA-3′
		R: 5′-TTCACTAGGGCCTGCTTGAT-3′
Fibroblast cell-related	Collagen I	F: 5′-CCGTGACCTCAAGATGTGCC-3′
		R: 5′-GCTCATACCTTCGCTTCCAA-3′
	α-SMA	F: 5′-CCGAGATCTCACCGACTACC-3′
		R: 5′-TCCAGAGCGACATAGCACAG-3′
	TIMP-1	F: 5′-TCCCCAGAAATCATCGAGAC-3′
		R: 5′-TCAGATTATGCCAGGGAACC-3′
EMT index	Snail	F: 5′-GAGGACAGTGGCAAAAGCTC-3′
		R: 5′-TCGGATGTGCATCTTCAGAG-3′
	Vimentin	F: 5′-AGATCGATGTGGACGTTTCC-3′
		R: 5′-CACCTGTCTCCGGTATTCGT-3′
	E-cadherin	F: 5′-GGGTTGTCTCAGCCAATGTT-3′
		R: 5′-CACCAACACACCCAGCATAG-3′
Target	HNF4α	F: 5′-AAATGTGCAGGTGTTGACCA-3′
		R: 5′-CACGCTCCTCCTGAAGAATC-3′
Control	β-actin	F: 5′-ACCCACACTGTGCCCATCTATG-3′
		R: 5′-AGAGTACTTGCGCTCAGGAGGA-3′

F and R stand for forward and reverse primers, respectively. HNF4α: Hepatocyte nuclear factor 4α.