Inflammatory microenvironment and expression of chemokines in hepatocellular carcinoma

Table 1 Observation of inflammatory cell phenotype in paired tumor and adjacent peritumoral samples, n = 3

Inflammatory cell	Tumor sample	Peritumoral samples	Mean difference	(95%CI)	P
T helper cell	12.09 ± 10.74	20.08 ± 13.31	7.53	(2.75-13.31)	0.005
Cytotoxic T cell	7.25 ± 8.07	6.05 ± 8.41	11.87	(21.60-2.14)	0.800
B cell	2.88 ± 6.36	4.31 ± 6.87	10.87	(19.60-4.07)	0.600
Activated T cell	2.12 ± 2.37	6.96 ± 5.17	5.10	(3.17-7.02)	0.005
NK cell	0.29 ± 1.30	2.30 ± 5.18	1.83	(0.21-3.45)	0.060
Macrophage	9.03 ± 9.97	19.76 ± 9.41	8.07	(4.00-12.13)	0.005
Mast cell	9.00 ± 5.35	10.44 ± 9.00	0.78	(21.07-3.09)	0.600
Neutrophil	1.56 ± 3.22	14.83 ± 14.76	13.28	(8.37-18.19)	0.005
Plasma cell	5.59 ± 7.11	6.50 ± 8.00	0.67	(20.16-4.14)	0.800
iNOS+ cells median (range)	0 (0-3)	10 (6-16)	-	-	0.005
Arginase I+ cells median (range)	1 (0-2)	0 (0-1)	-	-	0.005

Data are expressed as mean ± SD unless otherwise indicated. iNOS: Inducible nitric oxide synthase.

Table 2 Upregulated chemokine-related genes in tumor tissues compared with peritumoral tissues

Accession No.	Gene name	Gene symbol	Fold change
A01	C5aR	C5ar1	8.86
A02	CCR10	Ccbp2	5.39
A04	Scya11	Ccl11	97.95
A05	Scya12	Ccl12	4.75
A07	CKb11	Ccl19	71.66
A08	HC11	Ccl2	25.81
A11	CKb-6	Ccl24	17.46
B06	MRP-1	Ccl6	20.28
B07	MCP-3	Ccl7	22.47
B08	MCP-2	Ccl8	16.51
B09	MRP-2	Ccl9	20.72
B10	Cmkbr1	Ccr1	5.2
B11	Cmkbr9	Ccr10	2.01
C01	Ccr2a	Ccr2	14.64
C02	CKR3	Ccr3	14.14
C04	CD195	Ccr5	9.77
C05	CCR-6	Ccr6	4.4
C11	mcmklrl	Cmklr1	8.96
C12	Cklf	Cmtm2a	2.25
D01	Cklfsf3	Cmtm3	28.58
D02	Cklfsf4	Cmtm4	2.94
D03	Cklfsf5	Cmtm5	4.14
D04	Cklfsf6	Cmtm6	5.27
D05	Cxc3	Cx3cl1	32.58
D07	KC	Cxcl1	23.49
D08	C7	Cxcl10	3.15
D09	Cxc11	Cxcl11	4.84
D10	Pbsf	Cxcl12	246.94
D12	KS1	Cxcl14	323.14
E02	Zmynd15	Cxcl16	2.3
E04	Gm1960	Cxcl3	12.76
E05	LIX	Cxcl5	458.57
E09	Cd183	Cxxr3	12.42
E10	CD184	Cxcr4	3.16
E12	B0NZ0	Cxcr6	9.26
F01	Rdc1	Cxcr7	3.6
F02	CCBP1	Darc	68.4
F04	AI853548	Gpr17	7.55
F05	MOP1	Hifla	12.83
F07	KIAA4048	Il16	3.96
F09	Il-4	Il4	3.73
F10	Il-6	Il6	87.67
F11	CR3	Itgam	5.51
F12	2E6	Itgbz	15.7
G02	Crk1	Mapk14	6.74
G03	Cxcl4	Pf4	9.21
G04	Cxcl7	Ppbp	14.62
G06	Tgfb	Tgfb1	12.46
G07	Ly105	Tlr2	8.67
G08	Lps	Tlr4	10.41
G09	Tnfa	Tnf	2.37
G12	Ccxcr1	Xcr1	17.78
H02	beta2m	B2m	6.14
H03	Gapd	Gapdh	41.27
H04	Gur	Gusb	3.63

Table 3 Downregulated chemokine-related genes in tumor tissues compared with peritumoral tissues

Accession No.	Gene name	Gene symbol	Fold change
E07	I18ra	Cxcr1	-4.18
F06	Ifng	Ifg	-2.02
H01	β-actin	Actb	-2.80