Expression of circulating microRNA-20a and let-7a in esophageal squamous cell carcinoma

Table 1 Relative miR-20a and let-7a levels

Group	Age (yr)	miR-20a	let-7a
ESCC patients	60.48 ± 8.40	2.09 ± 1.39	0.53 ± 0.43
Healthy controls	61.72 ± 6.93	1.12 ± 0.68	1.13 ± 0.58
P-value	0.431	0.000	0.000

ESCC: Esophageal squamous cell carcinoma.

Table 2 Correlation between miR-20a and let-7a levels and clinicopathologic features in patients with esophageal squamous cell carcinoma

Characteristics	n	Fold change1
		miR-20a	let-7a
Gender
Male	46	1.61 (0.95-2.82)	0.33 (0.22-0.53)
Female	24	1.63 (1.33-2.93)	0.45 (0.29-0.89)
P-value		0.481	0.107
Age (yr)
≤ 60	37	1.62 (1.24-2.89)	0.39 (0.23-0.79)
> 60	33	1.62 (1.06-2.82)	0.35 (0.24-0.64)
P-value		0.463	0.900
Diameter of tumor
< 4 cm	36	1.51 (0.99-2.47)	0.40 (0.23-0.79)
≥ 4 cm	34	2.10 (1.33-3.08)	0.36 (0.24-0.62)
P-value		0.077	0.961
TNM stage
I/II	49	1.52 (0.95-2.80)	0.42 (0.28-0.88)
III/IV	21	2.04 (1.40-3.40)	0.24 (0.19-0.39)
P-value		0.118	0.009
Distant metastasis
M0	61	1.62 (1.10-2.82)	0.39 (0.24-0.79)
M1	9	1.64 (1.21-4.45)	0.35 (0.12-0.44)
P-value		0.300	0.174
Histologic grade
Well differentiated	25	1.50 (1.10-2.41)	0.45 (0.25-0.59)
Moderately differentiated	28	1.76 (1.04-2.82)	0.33 (0.26-0.97)
Poorly differentiated	17	1.75 (1.21-3.29)	0.30 (0.22-0.62)
P-value		0.632	0.849

¹Median of relative expression with 25^th-75^th percentile; Mann-Whitney U test waws performed for comparisons between two groups and Kruskall-Wallis test for comparisons among three groups.