Copyright
©The Author(s) 2015.
World J Gastroenterol. Apr 7, 2015; 21(13): 3960-3969
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3960
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3960
Primer | Sequence (5'→3') | Amplicon (bp) |
RT-PCR | ||
IRAK3-Forward | ATGCAGTGTAAGAAGCATTGGA | 247 |
IRAK3-Reverse | GCAGGTAGTGAATGGCTTTGG | |
GLOXD1-Forward | CCCTTCCTACCCGGCTTCA | 122 |
GLOXD1-Reverse | TGGAACCAGCGCAAAAGTGT | |
Pyrosequencing | ||
GLOXD1-Forward | GAAGGGAGGTTTAGTGTTTAAGGA | 242 |
GLOXD1-Reverse | AGCTGGACATCACCTCCCACAACGCCACCCCAACCAAAAACA | |
Universal primer | AGCTGGACATCACCTCCCACAACG-Biotin | |
Sequencing primer | AGGTTTAGTGTTTAAGGAT | |
MSP/Q-MSP | ||
IRAK3-Forward | AGGAGATCGTTTAGTCGTGGGGTAC | 110 |
IRAK3-Reverse | ACCTCTACGATAAAAACGAAACTACCG | |
IRAK3-Probe | CTACCGAAACAAACAAAATA | |
GLOXD1-Forward | AGGATGTGATTAGGCGTGAGGTTC | 122 |
GLOXD1-Reverse | AAAAAAACGAAACCCGTAACTCCG | |
GLOXD1-Probe | FAM-CGCTACTCTTTCCCC |
Characteristic | Cases |
Age, yr | 59 ± 14 |
Mean ± SD | |
Gender | |
Female | 94 |
Male | 66 |
Hepatitis | |
HBV-positive | 68 |
HCV-positive | 62 |
Double-negative | 30 |
Cirrhosis | |
No | 77 |
Yes | 80 |
Unknown | 3 |
Tumor size, cm | |
≤ 3 | 52 |
> 3 | 108 |
Nodule | |
Solitary | 98 |
Multiple | 62 |
AFP level, ng/mL | |
≤ 10 | 45 |
> 10 | 113 |
Unknown | 2 |
Stage | |
I | 60 |
II | 46 |
III | 47 |
IV | 7 |
Invasion | |
No | 85 |
Yes | 75 |
Recurrence | |
No | 58 |
Yes | 36 |
Unknown | 66 |
Survival | |
No | 71 |
Yes | 27 |
Unknown | 62 |
Methylation status | IRAK3, M-Index | P value | GLOXD1, M-Index | P value | ||
Characteristic | ≤8.90 | > 8.90 | ≤0.60 | > 0.60 | ||
Cases | 58 | 102 | 85 | 75 | ||
Age, yr | ||||||
≤ 59 | 27 | 47 | 1.00 | 45 | 29 | 0.08 |
> 59 | 31 | 55 | 40 | 46 | ||
Gender | ||||||
Female | 25 | 41 | 0.74 | 39 | 27 | 0.26 |
Male | 33 | 61 | 46 | 48 | ||
Hepatitis | ||||||
HBV-positive | 21 | 47 | 0.32 | 38 | 30 | 0.22 |
HCV-positive | 23 | 39 | 28 | 34 | ||
Double-negative | 14 | 16 | 19 | 11 | ||
Cirrhosis | ||||||
No | 33 | 44 | 0.07 | 46 | 31 | 0.15 |
Yes | 23 | 57 | 38 | 42 | ||
Unknown | 2 | 1 | 1 | 2 | ||
Tumor size, cm | ||||||
≤ 3 | 16 | 36 | 0.38 | 25 | 27 | 0.40 |
> 3 | 42 | 66 | 60 | 48 | ||
Nodule | ||||||
Solitary | 37 | 61 | 0.74 | 53 | 45 | 0.87 |
Multiple | 21 | 41 | 32 | 30 | ||
AFP level, ng/mL | ||||||
≤ 10 | 14 | 31 | 0.58 | 20 | 25 | 0.16 |
> 10 | 42 | 71 | 65 | 48 | ||
Unknown | 2 | 0 | 0 | 2 | ||
Stage | ||||||
I | 22 | 38 | 0.03 | 28 | 32 | 0.66 |
II | 23 | 23 | 26 | 20 | ||
III | 13 | 34 | 27 | 20 | ||
IV | 0 | 7 | 4 | 3 | ||
Invasion | ||||||
No | 30 | 55 | 0.87 | 39 | 46 | 0.06 |
Yes | 28 | 47 | 46 | 29 | ||
Recurrence | ||||||
No | 22 | 36 | 0.38 | 34 | 24 | 0.83 |
Yes | 10 | 25 | 19 | 16 | ||
Unknown | 26 | 41 | 32 | 35 | ||
Survival | ||||||
No | 25 | 46 | 0.47 | 38 | 33 | 0.26 |
Yes | 7 | 20 | 18 | 9 | ||
Unknown | 26 | 36 | 29 | 33 |
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Citation: Kuo CC, Shih YL, Su HY, Yan MD, Hsieh CB, Liu CY, Huang WT, Yu MH, Lin YW. Methylation of
IRAK3 is a novel prognostic marker in hepatocellular carcinoma. World J Gastroenterol 2015; 21(13): 3960-3969 - URL: https://www.wjgnet.com/1007-9327/full/v21/i13/3960.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i13.3960