Copyright
©The Author(s) 2015.
World J Gastroenterol. Apr 7, 2015; 21(13): 3876-3887
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3876
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3876
Table 1 Primer and shRNA sequences for the genes examined in the present study
| Genes | Analysis | 5'-Sequence-3' |
| FUT1 | RT-PCR | F: AGACTTTGCCCTGCTCACAC |
| R: TGAAGTTGGCCAGGTAGACAG | ||
| FUT2 | RT-PCR | F: CAGATGCCTTTCTCCTTTCC |
| R: ACTCCCACATGGCTTGAATC | ||
| FUT3 | RT-PCR | F: CTGGATCTGGTTCAACTTGG |
| R: CGGTAGGACATGGTGAGATTG | ||
| FUT4 | RT-PCR | F: GGGTTTGGATGAACTTCGAG |
| R: AGCCATAAGGCACAAAGACG | ||
| FUT8 | RT-PCR | F: ATCCTGATGCCTCTGCAAAC |
| R: GGGTTGGTGAGCATAAATGG | ||
| GMDS | RT-PCR | F: TGGAGGCTATGTGGTTGATG |
| R: CAAATTCCCGGACACTATGG | ||
| FX | RT-PCR | F: ACACGTCATCCATCTTGCTG |
| R: AGGACGTTGTCGTTCATGTG | ||
| SLC35C1 | RT-PCR | F: GGTGTGGCCTTCTACAATGTG |
| R: ATGATGATACCGCAGGTGAG | ||
| HNF4α | RT-PCR | F: CATCTTCTTTGACCCAGATGC |
| R: CGTTGATGTAGTCCTCCAAGC | ||
| IL-6 | RT-PCR | F: ATGCAATAACCACCCCTGAC |
| R: GCGCAGAATGAGATGAGTTG | ||
| RPL4 | RT-PCR | F: GACTTAACACACGAGGAGATGC |
| R: GCATGCTGTGCACATTTAGG | ||
| FUT1 | shRNA | GGTAATCAGATGGGACAGTAT |
| Negative control | shRNA | GGAATCTCATTCGATGCATAC |
| SLC35C1 | shRNA | CUACUUAUAGACCCAAUCATT |
| Negative control | shRNA | UCUUAAUCGCGUAUAAGGCTT |
- Citation: Terao N, Takamatsu S, Minehira T, Sobajima T, Nakayama K, Kamada Y, Miyoshi E. Fucosylation is a common glycosylation type in pancreatic cancer stem cell-like phenotypes. World J Gastroenterol 2015; 21(13): 3876-3887
- URL: https://www.wjgnet.com/1007-9327/full/v21/i13/3876.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i13.3876