Evaluation of specific fecal protein biochips for the diagnosis of colorectal cancer

Table 1 Information of colorectal cancer patients and healthy controls n (%)

	Colorectal cancer (n = 20)	Healthy controls (n = 20)
Gender
Male	10 (50)	14 (70)
Female	10 (50)	6 (30)
Age group (yr)
46-55	5 (25)	5 (25)
56-65	3 (15)	4 (20)
66-75	6 (30)	7 (35)
≥ 75	6 (30)	4 (20)
Location of tumor
Ascending colon	6 (30)	-
Transverse colon	1 (5)	-
Descending colon	6 (30)	-
Rectum	7 (35)	-
Tumor stage (TNM)
I	2 (10)	-
II	7 (35)	-
III	9 (45)	-
IV	2 (10)	-

Table 2 Data of the first five proteins after normalization

Name	C-Nor	N-Nor	C/N
Angiopoietin-2	263.60	6.89	38.27
MMP-10	223.58	10.14	22.05
FGF-23	345.69	22.60	15.3
Thrombopoietin	229.29	18.03	12.72
IL-13	243.38	21.05	11.56

N: Normal control group; C: Colorectal cancer group; MMP: Proteins of the matrix metalloproteinase; FGF: fibroblast growth factor-23; IL-13: Interleukin-13.

Table 3 Information of area under the curve and 95%CI of the seven proteins

Test result variable(s)	Area	SE	Asymptotic sig	Asymptotic 95%CI
				Lower bound	Upper bound
Calprotectin	1.000	0.000	0.000	1.000	1.000
M2-PK	0.933	0.039	0.000	0.856	1.009
Ang-2	1.000	0.000	0.000	1.000	1.000
MMP-10	0.991	0.010	0.000	0.972	1.011
FGF-23	0.994	0.008	0.000	0.979	1.010
TPO	1.000	0.000	0.000	1.000	1.000
IL-13	1.000	0.000	0.000	1.000	1.000

M2-PK: M2-pyruvatekinase; Ang-2: Angiopoietin-2; MMP-10: Proteins of the matrix metalloproteinase-10; TPO: Thrombopoietin; IL-13: Interleukin-13; FGF-23: Fibroblast growth factor-23.

Table 4 Cutoff value, 95%CI, P value of the protein concentration

Name	SE	mean	95%CI	Cutoff value	P value
Calprotectin-C	5.725	179.12	167.09-191.14	144.39	0.00
Calprotectin-N	2.906	116.08	109.95-122.21
M2-PK-C	1.516	141.15	137.97-144.34	114.49	0.00
M2-PK-N	0.725	100.19	98.66-101.72
Ang-2-C	0.627	86.46	85.14-87.78	69.62	0.00
Ang-2-N	0.324	55.85	55.16-56.53
MMP-10-C	4.505	518.37	508.91-527.84	440.41	0.00
MMP-10-N	2.267	348.61	343.82-353.89
FGF-23-C	0.607	72.84	71.56-74.11	56.33	0.00
FGF-23-N	0.304	48.55	47.91-49.19
TPO-C	0.118	19.76	19.51-20.01	13.08	0.00
TPO-N	0.069	7.39	7.24-7.53
IL-13-C	0.122	21.01	20.76-21.27	16.76	0.00
IL-13-N	0.064	12.99	12.85-13.12

N: Normal control group; C: Colorectal cancer group; M2-PK: M2-pyruvatekinase; Ang-2: Angiopoietin-2; MMP-10: Proteins of the matrix metalloproteinase-10; TPO: Thrombopoietin; IL-13: Interleukin-13; FGF-23: Fibroblast growth factor-23.